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首页> 外文期刊>American Journal of Translational Research >Effect of miRNA-136-targeted regulation of FGFR1 on proliferation and apoptosis of triple-negative breast cancer cells
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Effect of miRNA-136-targeted regulation of FGFR1 on proliferation and apoptosis of triple-negative breast cancer cells

机译：MiRNA-136针对性调节FGFR1对三阴性乳腺癌细胞增殖和凋亡的影响

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Purpose: This study was designed to investigate the effect of micro RNA-targeted regulation of FGFR1 on the proliferation and apoptosis of triple-negative breast cancer (TNBC) cells. Methods: TNBC (MAD-MB-231), three types of breast cancer (MCF10A, MCF7, ZR751) cell lines, and normal breast tissue cell lines were extracted. Real-time PCR was used to detect the expression of miRNA-136 in different types of breast cells. The MAD-MB-231 cell lines were transfected with miRNA-136 mimic by lipofection. The effects of miRNA-136 on FGFR1 expression and apoptosis rate of MAD-MB-231 cell lines were determined using western blotting. Results: miRNA-136 expression in TNBC cells was lower than that of controls, and was negatively correlated with TNM staging. miRNA-136 expression in MCF10A, MCF7, ZR751, and MAD-MB-231 cell lines was gradually decreased, and MCF10A expression in the other three cell lines was significantly higher than that of MAD-MB-231 cell line (P0.05). Transfection with miRNA-136 significantly reduced the proliferation rate of MAD-MB-231, and a higher concentration and longer duration exhibited a more pronounced inhibitory effect on proliferation (P0.05). Transfection with miRNA-136 significantly reduced FGFR1 expression in the MAD-MB-231 cell lines, without significantly affecting apoptosis. Conclusion: miRNA-136 shows a very low expression level in TNBC cells. Transfection with miRNA-136 can significantly inhibit the proliferation of TNBC cells by external transfection, and has little effect on cell apoptosis. This may be related to miRNA-mediated changes in FGFR1 protein expression.
机译：目的：本研究旨在探讨FGFR1对三阴性乳腺癌（TNBC）细胞增殖和凋亡的微RNA靶向调节的影响。方法：提取TNBC（MAD-MB-231），提取三种类型的乳腺癌（MCF10A，MCF7，ZR751）细胞系和正常乳房组织细胞系。实时PCR用于检测不同类型的乳腺细胞中miRNA-136的表达。 MAD-MB-231细胞系通过脂质纤维模拟MiRNA-136转染。使用Western印迹测定miRNA-136对FGFR1表达和Mad-MB-231细胞系细胞凋亡率的影响。结果：在TNBC细胞中的miRNA-136表达低于对照的表达，与TNM分期负相关。 MIRNA-136在MCF10A，MCF7，ZR751和MAD-MB-231细胞系中的表达逐渐降低，另外三种细胞系中的MCF10A表达明显高于MAD-MB-231细胞系（P <0.05） 。用miRNA-136转染显着降低了Mad-MB-231的增殖率，较高浓度和更长的持续时间表现出对增殖的更明显的抑制作用（P <0.05）。用miRNA-136转染显着降低了Mad-MB-231细胞系中的FGFR1表达，而不会显着影响细胞凋亡。结论：MiRNA-136显示TNBC细胞中的非常低的表达水平。用miRNA-136转染可以通过外部转染显着抑制TNBC细胞的增殖，对细胞凋亡几乎没有影响。这可能与FGFR1蛋白表达中的miRNA介导的变化有关。

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