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首页> 外文期刊>Egyptian Journal of Biological Pest Control >A new strain of Bacillus velezensis as a bioagent against Verticillium dahliae in cotton: isolation and molecular identification
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A new strain of Bacillus velezensis as a bioagent against Verticillium dahliae in cotton: isolation and molecular identification

机译：棉花芽孢杆菌的新菌株作为棉花棉花黄毛利菊酯的生物制造，分离和分子鉴定

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Verticillium dahliae is one of the most destructive pathogens in many cotton-growing countries around the globe. There is still the lack of high-resistant upland cotton cultivars against this pathogen, and the usage of fungicides is threatening the environment. Biocontrol is an environment-friendly and a green technique in plant protection. In this regard, the present work was aimed to investigate antagonistic bacterial strains and to introduce them as new bioinoculants against V. dahliae in cotton. Numerous bacteria were isolated from cotton roots, and their antagonism was examined against V. dahliae. Strain SZAD2 which was identified as Bacillus velezensis on the bases of 16S rRNA gene analysis had significantly high biocontrol potential both in plate assay and under greenhouse conditions. The biocontrol efficacy in seed treatment was 60.31%, while in the soil drench method, it was 79.19%. Transmission electron microscopy (TEM) analysis revealed that the strain systemically colonized the roots and lived within the intercellular space. Hydrogen peroxide staining exposed that the strain increased plant basal defense response by the accumulation of hydrogen peroxide (H2O2) in both roots and leaves. Furthermore, the bacterization of cotton roots triggered induced systemic resistance (ISR) in the roots and caused the activation of the antioxidant enzymes such as phenylalanine ammonia-lyase (PAL), polyphenol oxidase (PPO), peroxidase (POD), and phenol contents. Moreover, the antifungal extract produced by the strain contained the activity of both cellulase and chitinase, which were able to suppress fungal mycelia both in the presence and absence of cotton. Strain SZAD2 systemically colonized the plant, showed multiple modes of action against the pathogen which played a vital role in V. dahliae control, and is recommended for commercial exploration as a safe alternative bioinoculant instead of hazardous chemical fungicides.
机译：Verticillium Dahliae是全球许多棉花种植国家最具破坏性的病原体之一。仍然缺乏对该病原体的高耐高兴棉质品种，并且使用杀菌剂的用途威胁着环境。 Biocontrol是一种环保和植物保护的绿色技术。在这方面，目前的作品旨在调查拮抗细菌菌株并将其作为新的生物遗传体引入针对棉花的V.Ahliae的新生物遗传体。从棉状根中分离出许多细菌，对其对抗哈利亚的拮抗作用。在16S rRNA基因分析基础上鉴定为Bacillus Velezensis的菌株SzAd2在板测定和温室条件下具有显着高的生物控制潜力。种子处理中的生物防治疗效为60.31％，而在土壤浸液法中，为79.19％。透射电子显微镜（TEM）分析显示，应变系统地殖民地根系并在细胞间空间内居住。过氧化氢染色暴露在根系和叶片中的过氧化氢（H2O2）积累的植物基础防御响应增加。此外，棉状根的细菌触发诱导的全身性抗性（ISR）在根部中，并导致抗氧化酶如苯丙氨酸氨酶（PAL），多酚氧化酶（PPO），过氧化物酶（POD）和酚含量的激活。此外，该菌株产生的抗真菌提取物包含纤维素酶和胰蛋白酶酶的活性，其能够在存在和不存在棉花中抑制真菌菌丝体。菌株SZAD2全身殖民该植物，显示出多种对病原体的作用方式，在V. Dahliae对照中发挥了至关重要的作用，建议用于商业勘探作为一种安全的替代生物植物，而不是有害化学杀菌剂。

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