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首页> 外文期刊>Spanish Journal of Agricultural Research >Short communication: Pepino mosaic virus, a new threat for Serbia’s tomatoes
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Short communication: Pepino mosaic virus, a new threat for Serbia’s tomatoes

机译：短期通讯：百吉尼诺马赛克病毒，对塞尔维亚西红柿的新威胁

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Aim of study: To report the occurrence of Pepino mosaic virus (PepMV) on tomato in Serbia and to genetically characterize Serbian PepMV isolates. Area of study: Tomato samples showing virus-like symptoms were collected in the Bogojevce locality (Jablanica District, Serbia). Material and methods: Collected tomato samples were assayed by DAS-ELISA using antisera against eight economically important or quarantine tomato viruses. Three selected isolates of naturally infected tomato plants were mechanically transmitted to tomato ‘Novosadski jabu?ar’ seedlings. For confirmation of PepMV infection, RT-PCR was performed using specific primers PepMV TGB F/PepMV UTR R. Maximum-likelihood phylogenetic tree was constructed with 47 complete CP gene sequences of PepMV to determine the genetic relationship of Serbian PepMV isolates with those from other parts of the world. Main results: The results of DAS-ELISA indicated the presence of PepMV in all tested samples. Mechanically inoculated ‘Novosadski jabu?ar’ seedlings expressed yellow spots and light and dark green patches, bubbling, and curled leaves. All tested tomato plants were RT-PCR positive for the presence of PepMV. The CP sequence analysis revealed that the Serbian PepMV isolates were completely identical among themselves and shared the highest nucleotide identity of 95.1% (99.2% aa identity) with isolate from Spain (FJ263341). Phylogenetic analysis showed clustering of the Serbian PepMV isolates into CH2 strain, but they formed separate subgroup within CH2 strain. Research highlights: This is the first data of the presence of PepMV in protected tomato production in Serbia. Considering increased incidence and rapid spread in Europe, the presence of PepMV on tomato could therefore represent serious threat to this valuable crop in Serbia.
机译：研究的目的：在塞尔维亚报告番茄素脑马赛克病毒（Pepmv）的发生，并遗传表征塞尔维亚肽分离物。研究领域：在Bogojevce地区（Jaultanica区，塞尔维亚）收集了显示病毒样症状的番茄样本。材料和方法：Das-Elisa使用抗血清对八个经济上重要或检疫的番茄病毒进行测定，从而测定番茄样品。三种选定的天然感染的番茄植物的分离物机械地传递给番茄群岛的Novosadski Jabu？幼苗。为了确认PepmV感染，使用特异性引物肽TGB F / PEPMV UTR R.使用47个完整的PEPEMV的CP基因序列构建最大似然性系统的最大似然性，以确定塞尔维亚肽与其他人的遗传关系世界各地。主要结果：Das-ELISA的结果表明所有测试样品中的PEPMV存在。机械接种的'Novosadski Jabu吗？幼苗表示黄斑和暗绿色斑块，冒泡和卷曲的叶子。所有测试的番茄植物都是肽存在的RT-PCR阳性。 CP序列分析显示，塞尔维亚肽肽分离物在其周期中完全相同，并与来自西班牙的分离物（FJ263341）分离出95.1％（99.2％AA同一性）的最高核苷酸同一性。系统发育分析显示塞尔维亚肽分离物的聚类分离为CH2菌株，但它们在CH2菌株中形成了单独的亚组。研究亮点：这是塞尔维亚受保护的番茄生产中Pepmv存在的第一个数据。考虑到欧洲的发病率增加和快速蔓延，因此番茄肽的存在可能对塞尔维亚的这种有价值的作物表示严重威胁。

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