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Characterization of Fusarium sambucinum isolates associated with potato dry rot and evaluation of cultivar susceptibility and fungicides

机译:与马铃薯干腐蚀相关的镰刀菌Sambucinum分离物的特征及品种易感性和杀菌剂评价

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Potato dry rot disease ( Fusarium spp.) is one of the most important fungal storage rots on potato tubers after harvesting. The aims of this study were to identify the Fusarium species that cause dry rot in potato storages, evaluate the most virulent Fusarium species in vitro and in vivo for fungicide sensitivity and determine the susceptibility of the cultivars. In this context, as a result of surveys carried out in potato storages of ?demi? and Torbal? counties in ?zmir province in 2015-2016; Fusarium species obtained from diseased potato tubers were identified as Fusarium sambucinum, F. oxysporum, F. avenaceum and F. equiseti , using morphological and molecular methods. The most virulent species was determined according to the pathogenicity tests. To determine the susceptibility to disease, 13 potato cultivars, commonly stored in the region throughout the working period, were tested against the most virulent Fusarium species. The pathogenicity tests showed variation between the rank order of susceptibility of the cultivars to the most virulent pathogen. Fungicide sensitivity tests have been conducted with the most susceptible among these cultivars. There are not any plant protection products licensed against this disease in Turkey. On fungicide sensitivity tests, fludioxonil, flutolanil, thiophanate methyl, imazalil, fludioxonil + sedaxane, fluxapyroxad and tolclophos-methyl + thiram efficacy were investigated on the mycelial growth of F. sambucinum . In vitro assays; fludioxonil, fludioxonil + sedaxane and imazalil were the most effective fungicides preventing mycelial growth of the pathogen. In addition to chemicals that are effective in vitro tests, efficacy of Bacillus subtilis QST 713 strain 1.34%, Pseudomonas fluorescens strain Pf1 1.5% and 42% carboxylic acid were also evaluated. In vivo tests were conducted with Russet Burbank, Lady Olympia, Granola cultivars and fungicides were applied in two different methods including before and after inoculation. Results of this study indicated that the treatments with fludioxonil + sedaxane had the highest efficacy and treatments before inoculation was more effective.
机译:马铃薯干腐病(镰刀菌SPP)是收获后马铃薯块茎最重要的真菌储存腐烂之一。本研究的目的是鉴定镰刀腐烂在马铃薯储存中腐烂的镰刀菌物种,在体外评价最具毒性的镰刀菌物种,用于杀菌剂敏感性,并确定品种的易感性。在这种情况下,由于在戏法的土豆储存中进行的调查?和torbal?县在2015 - 2016年Zmir省;利用形态学和分子方法鉴定从患病的马铃薯块茎获得的镰刀菌毒物,F. oxysporum,F. Avenaceum和F. Equiseti。根据致病性测试确定最具毒性的物种。为了确定对疾病的敏感性,在整个工作期间通常储存在该地区的13种马铃薯品种,针对最致华的镰刀菌。致病性试验在最具毒性病原体中显示了培养易感性的级别阶数之间的变化。杀菌剂敏感性试验已经在这些品种中最易受影响。没有任何植物保护产品在土耳其持有这种疾病。研究F.Sambucinum的菌丝体生长,研究了杀菌剂敏感性试验,氟噻唑,氟甲基,硫氰酸甲基,咪唑,Fludioxonon + Sedaxane,浮动素瘤和甲基甲基+喉疗效。体外测定; Fludioxonil,Fludioxonil + Sedaxane和Imazalil是预防病原体菌丝体生长的最有效的杀菌剂。除了有效的体外测试的化学品外,还评估了枯草芽孢杆菌QST713菌株的疗效1.34%,荧光荧光菌株PF1 1.5%和42%羧酸。在体内测试用赤褐版伯班,夫人奥林匹亚,格兰诺拉麦片品种和杀菌剂以两种不同的方法应用,包括在接种之前和之后。该研究的结果表明,在接种之前,含有FludioXonil + Sedaxane的治疗具有最高的疗效和治疗方法更有效。

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