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首页> 外文期刊>The Journal of automatic chemistry >LC-MS/MS Method for Rapid Quantification of Progesterone in Rabbit Plasma and Its Application in a Pharmacokinetic Study of the Transdermal Formulation
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LC-MS/MS Method for Rapid Quantification of Progesterone in Rabbit Plasma and Its Application in a Pharmacokinetic Study of the Transdermal Formulation

机译：LC-MS / MS方法，用于快速定量兔血浆中黄体酮及其在透皮制剂的药代动力学研究中的应用

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摘要

A rapid and effective method using QuEChERS-based sample preparation procedure and liquid chromatography-tandem mass spectrometry (LC-MS/MS) analysis has been developed and validated to determine progesterone in rabbit plasma. The analyte was extracted from plasma by acetonitrile with phase partitioning by a mixture of magnesium sulfate and sodium chloride. The supernatant was then directly injected into LC-MS/MS in a positive electrospray ionization mode and quantified using progesterone-d9 as the internal standard. The method linearity was in the range from 1?ng/mL (LOQ) to 200?ng/mL. Method recovery was from 86.0% to 103%, and repeatability was lower than 5.5%. The plasma sample was stable for 12 weeks stored at 18?±?2°C. This method was applied to quantify progesterone in rabbit plasma in a pharmacokinetic study of two transdermal formulations: a reference drug and a eutectic-hydrogel system. The data indicate that the eutectic-hydrogel system's bioavailability was 1.5 times better than that of the reference drug, and the transdermal system is a potential drug delivery system for progesterone.
机译：已经开发出并验证了使用基于QueCher的样品制备程序和液相色谱 - 串联质谱（LC-MS / MS）分析的快速有效的方法，以确定兔血浆中的孕激素。通过乙腈从血浆中从等离子体中萃取分析物，通过硫酸镁和氯化物的混合物进行相位分配。然后将上清液直接注射到正电喷雾电离模式中以LC-MS / MS注入LC-MS / MS中，并使用孕酮-D9定量作为内标。该方法线性度在1？Ng / ml（LOQ）至200≤ng/ ml的范围内。方法回收率为86.0％至103％，重复性低于5.5％。等离子体样品稳定为18℃储存12周，±2℃。应用该方法以在两种透皮制剂的药代动力学研究中量化兔血浆中的孕酮：参考药物和共晶水凝胶系统。数据表明，共晶 - 水凝胶系统的生物利用度比参考药物的生物利用度好1.5倍，透皮系统是孕酮的潜在药物递送系统。

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