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首页> 外文期刊>The Journal of automatic chemistry >Chemical Comparison of White Ginseng before and after Extrusion by UHPLC-Q-Orbitrap-MS/MS and Multivariate Statistical Analysis
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Chemical Comparison of White Ginseng before and after Extrusion by UHPLC-Q-Orbitrap-MS/MS and Multivariate Statistical Analysis

机译：UHPLC-Q-Orbitrap-MS / MS和多变量统计分析挤出前后白参化学比较和挤出

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摘要

Ultrahigh-performance liquid chromatography Quadrupole-Orbitrap tandem mass spectrometry (UHPLC-Q-Orbitrap-MS/MS) was used to compare the composition of ginsenosides in white ginseng (WG) and extruded white ginseng (EWG). A total of 45 saponins, including original neutral ginsenosides, malonyl-ginsenosides, and chemical transformation of ginsenosides, were successfully identified in both WG and EWG. Multivariate statistical analyses including supervised orthogonal partial least squared discrimination analysis (OPLS-DA) and hierarchical clustering analysis (HCA) were used to analyze components of white ginseng before and after extrusion. As a result, three ginsenosides (malonyl (M)-Rb1, M-Rb2, and M-Rc) were found to be increased in WG, while three ginsenosides (Rb2, Rc, and Rg1) were elevated in EWG. In the OPLS-DA S-plot, the different compositions of ginsenoside that were distinguished between WG and EWG were screened out. Experimental results indicate that the UHPLC-Q-Orbitrap-MS/MS is a useful tool to characterize variations of ginsenosides in WG and EWG.
机译：超高效液相色谱法谐波 - 壁图串联质谱（UHPLC-Q-Orbitrap-MS / MS）用于比较白人人参（WG）中人参皂苷的组成，并挤出白色人参（EWG）。在WG和EWG中成功地鉴定了总共45个皂苷，包括原始中性人参皂苷，包括原始中性人参皂苷，丙二酰鎓皂苷和人参皂苷的化学转化。包括监督正交部分最小二乘辨别分析（OPLS-DA）和分层聚类分析（HCA）的多变量统计分析用于分析挤出前后白参的组分。结果，发现三个人参皂苷（（M）-RB1，M-RB2和M-RC）在WG中增加，而在EWG中升高了三个人参皂苷（RB2，RC和RG1）。在OPLS-DA S-PLOT中，筛选出在WG和EWG之间区分的不同组成的人参皂苷。实验结果表明，UHPLC-Q-ORBITRAP-MS / MS是一种有用的工具，其表征WG和EWG中的人参皂苷的变化。

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