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首页> 外文期刊>The Journal of automatic chemistry >Separation and Determination of Paraquat and Diquat in Human Plasma and Urine by Magnetic Dispersive Solid Phase Extraction Coupled with High-Performance Liquid Chromatography
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Separation and Determination of Paraquat and Diquat in Human Plasma and Urine by Magnetic Dispersive Solid Phase Extraction Coupled with High-Performance Liquid Chromatography

机译：用高效液相色谱耦合磁性色散固相萃取分离和测定人血浆和尿液中尿液和尿液

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摘要

A magnetic dispersive solid phase extraction method coupled with high-performance liquid chromatography was proposed for the simultaneous separation and determination of paraquat (PQ) and diquat (DQ) in human plasma and urine samples. Based on the reduction of PQ and DQ to a blue radical and yellow-green radical by sodium dithionite in an alkaline medium, a fast colorimetric method was also developed for the fast detection of PQ or DQ. In this paper, CoFe_(2)O_(4)@SiO_(2) magnetic nanoparticles were used as the adsorbent for the magnetic dispersive solid phase extraction of paraquat and diquat, and these two analytes were found to be eluted directly from the adsorbent by NaOH solution. The main factors affecting the extraction efficiency including amount of extractant, extraction time, sample volume, sample solution pH, and elution volume were optimized. Under the optimized experimental conditions, the calibration curve was linear at a concentration range of 28.5–570.2? μ g/L, and the correlation coefficient of paraquat and diquat was 0.9986 and 0.9980, respectively. The limits of detection of paraquat and diquat were 4.5? μ g/L and 4.3? μ g/L. The proposed MSPE-HPLC method was successfully applied to the detection of the paraquat and diquat in human plasma and urine with satisfied recoveries of PQ and DQ in the range of 87.5%–98.7%.
机译：提出了一种与高性能液相色谱偶联的磁性色散固相提取方法，用于同时分离和测定人血浆和尿液样品中的百草枯（PQ）和Diquat（DQ）。基于PQ和DQ的降低通过二硫酸钠在碱性介质中的二硫酸钠和黄绿色的基础上，还开发了快速比色法用于快速检测PQ或DQ。在本文中，CoFe_（2）O_（4）@siO_（2）致磁性纳米粒子用作百草枯和直脂的磁性色散固相萃取的吸附剂，并发现这两种分析物直接从吸附剂中洗脱NaOH解决方案。优化了影响提取效率，提取时间，样品体积，样品溶液pH和洗脱体积的提取效率的主要因素。在优化的实验条件下，校准曲线在28.5-570.2的浓度范围内是线性的？ μg/ l，百草枯和肝炎的相关系数分别为0.9986和0.9980。检测百草枯和肝脏的极限为4.5？ μg / l和4.3？ μg/升。所提出的MSPE-HPLC方法成功地应用于人血浆和尿液中的百草枯和肝炎的检测，并满足PQ和DQ的回收率为87.5％-98.7％。

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