首页> 外文期刊>The Journal of automatic chemistry >Flow-Batch Sample Preparation for Fractionation of the Stress Signaling Phytohormone Salicylic Acid in Fresh Leaves
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Flow-Batch Sample Preparation for Fractionation of the Stress Signaling Phytohormone Salicylic Acid in Fresh Leaves

机译:用于在新鲜叶片中的应力信号传导植物激素水杨酸的分馏的流动分批样品制剂

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Salicylic acid (SA) is an important stress signaling phytohormone and plays an essential role in physiological processes in plants. SA fractionation has been carried out batchwise, which is not compatible with the high analytical demand in agronomical studies and increases susceptibility to analytical errors. In this context, a novel flow-batch sample preparation system for SA fractionation on fresh plant leaves was developed. It was based on microwave-assisted extraction with water and conversion of the conjugated species to free SA by alkaline hydrolysis. Free and total SA were quantified by fluorimetry after separation by sequential injection chromatography in a C18 monolithic column. The proposed procedure is directly applicable to plant leaves containing up 16?mg?kg~(?1) SA, with a limit of detection of 0.1?mg?kg~(?1) of SA, coefficient of variation of 3.0% ( n ?=?10), and sampling rate of 4 samples h~(?1). The flow-batch sample preparation system was successfully applied to SA fractionation in sugarcane, corn, and soybean leaves without clogging or increasing in backpressure. The proposed approach is simple, less time-consuming, and more environmentally friendly in comparison to batchwise procedures.
机译:水杨酸(SA)是一种重要的应力信号传导植物激素,在植物中的生理过程中起重要作用。 SA分馏已经分批进行,这与农艺研究中的高分析需求不相容,并增加对分析误差的易感性。在这种情况下,开发了一种新的新型流动分批样品制备系统,用于新鲜植物叶片上的SA分馏。它基于微波辅助提取用水和共轭物种转化为通过碱水解的游离SA。通过C18单片柱中的顺序注射色谱分离后,通过荧光测量来定量自由和总SA。所提出的程序直接适用于含有16Ω·kg〜(α1)sa的植物叶,其检测限为0.1Ω·kg〜(α1),变异系数为3.0%(n ?=?10),4个样品H〜(?1)的采样率。流动分批样品制备系统成功地应用于甘蔗,玉米和大豆叶中的SA分馏而不堵塞或增加背压。与分批程序相比,所提出的方法很简单,耗时,更环保。

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