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首页> 外文期刊>The Journal of automatic chemistry >Identification and Analysis of Components in Yizhi Granule and Cynomolgus Monkey Plasma after Oral Administration by UPLC/ESI-Q-TOF MS and Their Protective Effects on PC12 Cells
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Identification and Analysis of Components in Yizhi Granule and Cynomolgus Monkey Plasma after Oral Administration by UPLC/ESI-Q-TOF MS and Their Protective Effects on PC12 Cells

机译：UPLC / ESI-Q-TOF MS在口服施用后伊智颗粒和猴猴血浆组分的鉴定及分析及其对PC12细胞的保护作用

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Yizhi Granule (YZG) is a health food containing six traditional Chinese medicines (TCMs). It improves memory barriers in rat experiments. Here, we describe the first fast and sensitive ultraperformance liquid chromatography/electrospray ionization quadrupole time-of-flight mass spectrometry (UPLC/ESI-Q-TOF MS) method for analyzing YZG in plasma. We used this technique for studies in cynomolgus monkey plasma. By comparing retention time, MS, and MS/MS data of reference compounds, 70 compounds were detected in YZG. Of these, 63 were identified including 60 saponins, 2 flavones, and 1 methyl ester. There were 33 saponins, 1 flavone, and 1 methyl ester in the plasma. Next, to study the therapeutic properties of YZG, the neuroprotective effect of some of the absorbed components was evaluated using PC12 cell damage caused by the A β _(25–35) model. The results showed that 9 compounds protect PC12 cells from A β _(25–35) with cell viability (%) of 111.00?±?8.12 (G-Rb1), 102.20?±?4.22 (G-Rb2), 100.34?±?6.47 (G-Rd), 102.83?±?2.10 (G-Re), 101.68?±?7.64 (NG-Fa), 101.19?±?7.83 (NG-R1), 102.53?±?0.55 (NG-R2), 106.88?±?4.95 (gypenoside A), and 103.95?±?4.11 (gypenoside XLIX), respectively, versus the control group (87.51?±?6.59). These results can reveal the real pharmacodynamic basis of YZG and provide a theoretical basis for subsequent studies. It can also provide some references for the research of Alzheimer's disease.
机译：Yizhi颗粒（YZG）是一种含有六种中药（TCMS）的健康食品。它改善了大鼠实验中的记忆障碍。这里，我们描述了第一快速敏感的超细液相色谱/电喷雾电离四极型飞行时间质谱（UPLC / ESI-Q-TOF MS）方法，用于分析等离子体中的YZG。我们利用这种技术在Cynomolgus猴子血浆中研究。通过比较参考化合物的保留时间，MS和MS / MS数据，在yzg中检测到70种化合物。其中，鉴定了63种，包括60个皂苷，2黄酮和1个甲酯。血浆中有33个皂苷，1强酮和1个甲酯。接下来，为了研究YZG的治疗性质，使用由β_（25-35）模型引起的PC12细胞损伤来评估一些吸收组分的神经保护作用。结果表明，9种化合物保护PC12细胞保护PC12细胞与β_（25-35）的细胞活力（％）为111.00？±8.12（G-RB1），102.20？±4.22（G-RB2），100.34？± ？6.47（G-RD），102.83？±2.10（G-RE），101.68？±7.64（NG-FA），101.19？±α？7.83（NG-R1），102.53？±？0.55（NG-R2 ），106.88？±4.95（粒皂苷A）和103.95？±4.11（肉桂糖苷XLIX）与对照组（87.51？±6.59）。这些结果可以揭示yzg的真正药效基础，为后续研究提供了理论依据。它还可以为阿尔茨海默病的疾病提供一些参考。

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