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首页> 外文期刊>The Journal of automatic chemistry >Integrating Study on Qualitative and Quantitative Characterization of the Major Constituents in Shuanghuanglian Injection with UHPLC/Q-Orbitrap-MS and UPLC-PDA
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Integrating Study on Qualitative and Quantitative Characterization of the Major Constituents in Shuanghuanglian Injection with UHPLC/Q-Orbitrap-MS and UPLC-PDA

机译：uhplc / q-orbitrap-ms和UpLC-PDA的双黄连注射液主要成分定性和定量表征研究

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摘要

As a clinically effective traditional Chinese medicine injection (TCMI), Shuanghuanglian injection (SHLI) is widely used in the treatment of upper respiratory tract infection and pneumonia. However, the shortage of quality analysis is a limitation that remains to be improved in the clinical application of SHLI. In this study, taking advantage of ultra-high-performance liquid chromatography tandem Q-Exactive Orbitrap high-resolution mass spectrometry (UHPLC/Q-Orbitrap-MS), 31 chemical components (eight organic acids, eleven flavonoids, five iridoid glycosides, four phenylethanoid glycosides, and three lignans) in SHLI were characterized, among which 22 components were unambiguously identified by reference compounds. The brief prediction results of network pharmacology indicated that the 22 targeted components may have anti-inflammatory, antibacterial, antiviral, and immunomodulatory activities. Using multiwavelength switching method, the 22 targeted components were quantified by ultra-performance liquid chromatography with photodiode array detector (UPLC-PDA) after the methodological validation. Based on the successfully established method, the total content of 22 components in 20 batches of SHLIs was efficiently determined with a slight variation between 10.25 and 11.28?mg/mL, which accounted for 38.7% in total solid of SHLI. This study performed a reliable chemical identification and provided a rapid and effective method for quality analysis, which contributed to the in-depth investigation and application of SHLI.
机译：作为一种临床有效的中药注射（TCMI），双号林氏注射（SHLI）广泛用于治疗上呼吸道感染和肺炎。然而，质量分析的短缺是在SHLI的临床应用中仍有待改善的限制。在本研究中，利用超高效液相色谱串联Q-辐射锻炼高分辨率质谱（UHPLC / Q-Orbitrap-MS），31种化学成分（八种有机酸，11种黄酮，五种氧化甘油酯，四个SHLI中的苯乙醇糖苷和三个木质素）的特征在于，其中22种组分通过参考化合物明确鉴定。网络药理学的简要预测结果表明，22种靶向组分可具有抗炎，抗菌，抗病毒和免疫调节活性。使用多波长切换方法，在方法验证之后通过超高性能液相色谱法量化22个靶向组分。基于成功建立的方法，在10.25和11.28×mg / ml之间有效地确定了20批Shlis中的22种组分的总含量，其占SHLI的总固体的38.7％。该研究进行了可靠的化学鉴定，并为质量分析提供了快速有效的方法，这有助于Shli的深入调查和应用。

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