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首页> 外文期刊>Applied Microbiology >Cyclic-di-GMP Regulates the Quorum-Sensing System and Biocontrol Activity of Pseudomonas fluorescens 2P24 through the RsmA and RsmE Proteins
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Cyclic-di-GMP Regulates the Quorum-Sensing System and Biocontrol Activity of Pseudomonas fluorescens 2P24 through the RsmA and RsmE Proteins

机译：Cyclic-Di-GMP通过RSMA和RSME蛋白调节假单胞菌荧光荧光荧光笔2P24的批量传感系统和生物防治活性

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Pseudomonas fluorescens 2P24 is a rhizosphere bacterium that protects many crop plants against soilborne diseases caused by phytopathogens. The PcoI/PcoR quorum-sensing (QS) system and polyketide antibiotic 2,4-diacetylphloroglucinol (2,4-DAPG) are particularly relevant to the strain’s biocontrol potential. In this study, we investigated the effects of c-di-GMP on the biocontrol activity of strain 2P24. The expression of the Escherichia coli diguanylate cyclase (YedQ) and phosphodiesterase (YhjH) in P. fluorescens 2P24 significantly increased and decreased the cellular concentration of c-di-GMP, respectively. The production of the QS signals N -acyl homoserine lactones (AHLs) and 2,4-DAPG was negatively regulated by c-di-GMP in 2P24. The regulatory proteins RsmA and RsmE were positively regulated by c-di-GMP. Genomic analysis revealed that 2P24 has 23 predicted proteins that contain c-di-GMP-synthesizing or -degrading domains. Among these proteins, C0J56_12915, C0J56_13325, and C0J56_27925 contributed to the production of c-di-GMP and were also involved in the regulation of the QS signal and antibiotic 2,4-DAPG production in P. fluorescens . Overexpression of C0J56_12915, C0J56_13325, and C0J56_27925 in 2P24 impaired its root colonization and biocontrol activities. Taken together, these results demonstrated that c-di-GMP played an important role in fine-tuning the biocontrol traits of P. fluorescens .IMPORTANCE In various bacteria, the bacterial second messenger c-di-GMP influences a wide range of cellular processes. However, the function of c-di-GMP on biocontrol traits in the plant-beneficial rhizobacteria remains largely unclear. The present work shows that the QS system and polyketide antibiotic 2,4-DAPG production are regulated by c-di-GMP through RsmA and RsmE proteins in P. fluorescens 2P24. The diguanylate cyclases (DGCs) C0J56_12915, C0J56_13325, and C0J56_27925 are especially involved in regulating the biocontrol traits of 2P24. Our work also demonstrated a connection between the Gac/Rsm cascade and the c-di-GMP signaling pathway in P. fluorescens .
机译：假单胞菌荧光荧光笔2P24是根际细菌，可保护许多作物植物免受由植物病原体引起的土壤造成的疾病。 PCOI / PCOR批量传感（QS）系统和聚酮化抗生素2,4-二乙酰乙酰氯葡糖醇（2,4-DAPG）与菌株的生物控制潜力特别相关。在这项研究中，我们研究了C-Di-GMP对菌株2p24的生物控制活性的影响。 P.荧光型2P24中的大肠杆菌二胍环化环酶（YEDQ）和磷酸二酯酶（YHJH）的表达分别显着增加和降低了C-DI-GMP的细胞浓度。通过2p24中的C-Di-GMP产生QS信号N-酰基均菌氨酸内酯（AHL）和2,4- DAPG的产生。调节蛋白RSMA和RSME由C-DI-GMP积极调节。基因组分析表明，2P24具有23个预测蛋白质，其含有C-Di-GMP合成或-Degrading结构域。在这些蛋白质中，COJ56_12915，COJ56_13325和COJ56_27925有助于生产C-DI-GMP，并且还涉及在P.荧光的QS信号和抗生素2,4-DAPG生产的调节。 2P24中C0J56_12915，C0J5_13325和C0J56_27925的过表达损害其根部定植和生物控制活动。这些结果表明，C-Di-GMP在微调P.荧光的生物控制性状性质中发挥了重要作用。分类在各种细菌中，细菌第二信使C-Di-GMP影响各种细胞过程。然而，C-Di-GMP在植物有益流虫中的生物控制性状的功能仍然很大程度上尚不清楚。本作者表明，QS系统和聚酮化抗生素2,4-DAPG生产由C-DI-GMP通过RSMA和RSME蛋白在P.荧光2P24中调节。 Diguanyl酸酯环酶（DGCS）CO 956_12915，COJ56_13325和COJ56_27925尤其参与调节2P24的生物控制性特征。我们的作品还展示了GAC / RSM级联和P.荧光的C-DI-GMP信号通路之间的联系。

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