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首页> 外文期刊>Applied Microbiology >Fungal-Associated Molecules Induce Key Genes Involved in the Biosynthesis of the Antifungal Secondary Metabolites Nunamycin and Nunapeptin in the Biocontrol Strain Pseudomonas fluorescens In5
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Fungal-Associated Molecules Induce Key Genes Involved in the Biosynthesis of the Antifungal Secondary Metabolites Nunamycin and Nunapeptin in the Biocontrol Strain Pseudomonas fluorescens In5

机译：真菌相关分子诱导涉及抗真菌次生代谢物的生物合成的关键基因，在生物控制菌株假单胞菌中荧光荧光蛋白和Nunapeptin。

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Pseudomonas fluorescens In5 synthesizes the antifungal cyclic lipopeptides (CLPs) nunamycin and nunapeptin, which are similar in structure and genetic organization to the pseudomonas-derived phytotoxins syringomycin and syringopeptin. Regulation of syringomycin and syringopeptin is dependent on the two-component global regulatory system GacS-GacA and the SalA, SyrF, and SyrG transcription factors, which activate syringomycin synthesis in response to plant signal molecules. Previously, we demonstrated that a specific transcription factor, NunF, positively regulates the synthesis of nunamycin and nunapeptin in P. fluorescens In5 and that the nunF gene is upregulated by fungal-associated molecules. This study focused on further unravelling the complex regulation governing CLP synthesis in P. fluorescens In5. Promoter fusions were used to show that the specific activator NunF is dependent on the global regulator of secondary metabolism GacA and is regulated by fungal-associated molecules and low temperatures. In contrast, GacA is stimulated by plant signal molecules leading to the hypothesis that P. fluorescens is a hyphosphere-associated bacterium carrying transcription factor genes that respond to signals indicating the presence of fungi and oomycetes. Based on these findings, we present a model for how synthesis of nunamycin and nunapeptin is regulated by fungal- and oomycete-associated molecules.IMPORTANCE Cyclic lipopeptide (CLP) synthesis gene clusters in pseudomonads display a high degree of synteny, and the structures of the peptides synthesized are very similar. Accordingly, the genomic island encoding the synthesis of syringomycin and syringopeptin in P. syringae pv. syringae closely resembles that of P. fluorescens In5, which contains genes coding for synthesis of the antifungal and anti-oomycete peptides nunamycin and nunapeptin, respectively. However, the regulation of syringomycin and syringopeptin synthesis is different from that of nunamycin and nunapeptin synthesis. While CLP synthesis in the plant pathogen P. syringae pv. syringae is induced by plant signal molecules, such compounds do not significantly influence synthesis of nunamycin and nunapeptin in P. fluorescens In5. Instead, fungal-associated molecules positively regulate antifungal peptide synthesis in P. fluorescens In5, while the synthesis of the global regulator GacA in P. fluorescens In5 is positively regulated by plant signal molecules but not fungal-associated molecules.
机译：荧光荧光素In5合成抗真菌环脂肽（CLPS）鼻霉素和宁肽，其在结构和遗传组织中与假单胞菌衍生的植物毒素掺入霉素和槲皮素相似。掺量霉素和槲皮素的调节依赖于双组分全局调节系统GACS-GACA和SALA，SYRF和SYRG转录因子，其响应于植物信号分子而激活读数霉素合成。以前，我们证明了特定的转录因子，NUNF，积极地调节鼻霉素和Nunapeptin的合成荧光素In5，并且通过真菌相关分子上调NUNF基因。该研究的重点是进一步解开CLP合成中的复杂调节荧光型In5。推动者融合用于表明特定的活化剂NUNF依赖于次生新陈代谢Gaca的全局调节剂，并受真菌相关分子和低温调节。相反，通过植物信号分子刺激Gaca，该植物信号分子导致P.荧光型是含有杂体相关的细菌的转录因子基因，该转录因子基因响应表明存在真菌和卵菌的存在。基于这些发现，我们提出了一种模型，用于如何通过真菌和oomycete相关分子对Nunamycin和Nunapeptin合成的模型.Pseudomonads中的循环脂肽（CLP）合成基因簇显示出高度的同步，以及结构合成的肽非常相似。因此，在P.Syringae PV中编码划艇霉素和槲皮素合成的基因组岛。 Syresaae非常类似于P.荧光型In5，其含有编码用于合成抗真菌和抗燕麦肽鼻霉素和宁肽蛋白的基因。然而，司霉素和槲皮素合成的调节与尼霉素和宁蛋白合成的含量不同。而CLP在植物病原体P.Syringae PV中的合成。通过植物信号分子诱导含油，这些化合物不会显着影响Nunamycin和Nunapeptin的合成荧光素In5。相反，真菌相关分子在P.荧光型中呈正调节抗真菌肽合成，而P.荧光荧光型Min5的全局调节剂Gaca的合成是通过植物信号分子的阳性调节，而不是真菌相关的分子。

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