...
首页> 外文期刊>Applied Microbiology >Primary and Secondary Succession Mediate the Accumulation of Biogenic Amines during Industrial Semidry Chinese Rice Wine Fermentation
【24h】

Primary and Secondary Succession Mediate the Accumulation of Biogenic Amines during Industrial Semidry Chinese Rice Wine Fermentation

机译:初级和二次继承在工业半水稻葡萄酒发酵过程中介导生物胺的积累

获取原文
           

摘要

The use of exogenous functional microorganisms to regulate biogenic amine (BA) content is a common approach in fermentation systems. Here, to better understand the microbial traits of succession trajectories in resource-based and biotic interference systems, the BA-related primary and secondary succession were tracked during industrial semidry Chinese rice wine (CRW) fermentation. Dominant abundance and BA-associated microbial functionality based on phylogenetic investigation of communities by reconstruction of unobserved states (PICRUSt) indicated that Citrobacter , Acinetobacter , Lactobacillus , Exiguobacterium, Bacillus, Pseudomonas , and Enterobacter spp. prominently contributed to the decarboxylase gene family in CRW. The expression levels of tyrosine decarboxylase ( tyrDC ), ornithine decarboxylase ( odc ), and agmatine deiminase ( aguA ) genes were assessed by quantitative PCR (qPCR). The transcription levels of these genes did not correlate with the BA formation rate during postfermentation, indicating that acidification and carbon source depletion upregulated the expression and microbes launch the dormancy strategy to respond to unfavorable conditions. Furthermore, microbial interference with CRW fermentation by Lactobacillus plantarum (ACBC271) and Staphylococcus xylosus (CGMCC1.8382) coinoculated at a ratio of 1:2 exhibited the best synergetic control of BA content. Spearman correlations revealed that Lactobacillus and Staphylococcus exhibited influence on BA-associated microbiota (|ρ| > 0), Exiguobacterium and Pseudomonas were strongly suppressed by Lactobacillus (ρ = ?0.867 and ρ = ?0.782, respectively; P ?
机译:使用外源性官能微生物来调节生物胺(BA)含量是发酵系统中的常见方法。这里,为了更好地了解资源和生物干扰系统中连续轨迹的微生物性状,在工业半稻葡萄酒(CRW)发酵期间跟踪了BA相关的主要和二次继承。基于未观察状态的重建(Picrust)基于群体系统发育调查的主导丰度和BA相关的微生物功能表明,涂膜杆菌,传导杆菌,乳酸杆菌,外杆菌,芽孢杆菌,假单胞菌和肠杆菌SPP。突出地促成CrW中的脱羧酶基因家族。通过定量PCR(QPCR)评估酪氨酸脱羧酶(TyrDC),鸟氨酸脱羧酶(ODC)和胍丁胺酰基酶(AGUA)基因的表达水平。这些基因的转录水平与介面期间的BA形成速率没有相关,表明酸化和碳源耗竭上调,表达和微生物发射休眠策略以应对不利条件。此外,用乳杆菌(ACBC271)(ACBC271)和葡萄球菌(CGMCC1.8382)以1:2的比例进行微生物干扰,表现出BA含量的最佳协同控制。 Spearman相关表明,乳酸杆菌和葡萄球菌表现出对BA相关的微生物菌(|ρ10)的影响,乳酸杆菌强烈抑制了癫痫术和假单胞菌(ρ= 0.867和ρ= 0.782),P?<0.05),葡萄球菌在植入的1:2组中显示出对乳酸杆菌(ρ=β0115)和柠檬杆菌(ρ=→0.188)的最强的抑制作用。外源添加菌株对特定细菌的高抑制作用呈现了获得的BA相关贡献者的证据。总体而言,这项工作对依赖于食物微生物生态的资源使用和功能微生物群的微生物性状的重要洞察力。管理物质使用和微生物相互作用的移位模式是微生物学和生态学的迁移模式。初级和次生微生物连续的分析允许随着时间的推移或在干扰后确定分类分类多样性,社区特征和功能转换。 BA生成动力学和资源消耗的模式,功能性偏见预测和微生物相互作用是阐明的微生物系统的平衡机理。干扰后次要继承触发资源使用的变化,这反过来影响主要继承和新陈代谢。在这项研究中,随着次要继承策略的干扰下发生的外源微生物的功能潜力,包括重新平衡和休眠,最终减少了BA积累。因此,这种继承系统可以促进关于依赖于自然生态系统中发生的资源使用和微生物相互作用的微生物性状的基本问题。

著录项

相似文献

  • 外文文献
  • 中文文献
  • 专利
获取原文

客服邮箱:kefu@zhangqiaokeyan.com

京公网安备:11010802029741号 ICP备案号:京ICP备15016152号-6 六维联合信息科技 (北京) 有限公司©版权所有
  • 客服微信

  • 服务号