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首页> 外文期刊>Applied Microbiology >Environmental Presence and Genetic Characteristics of Carbapenemase-Producing Enterobacteriaceae from Hospital Sewage and River Water in the Philippines
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Environmental Presence and Genetic Characteristics of Carbapenemase-Producing Enterobacteriaceae from Hospital Sewage and River Water in the Philippines

机译：菲律宾医院污水和河水生产碳碱酶机肠杆菌区的环境存在和遗传特征

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This study aimed to evaluate the prevalence and genetic characteristics of carbapenemase-producing Enterobacteriaceae (CPE) in hospital sewage and river water in the Philippines, which has a typical tropical maritime climate. We collected 83 water samples from 7 hospital sewage and 10 river water sites. CPE were identified using CHROMagar mSuperCARBA, and Gram-negative strains were identified using matrix-assisted laser desorption ionization–time of flight mass spectrometry (MALDI-TOF MS) or 16S rRNA gene sequencing. Resistance genes in Enterobacteriaceae strains were identified using PCR and DNA sequencing, and transferability of carbapenemase genes from the CPE was investigated with conjugation experiments. Genotyping was performed using multilocus sequence typing (MLST) for Escherichia coli and Klebsiella pneumoniae . Out of 124 Enterobacteriaceae isolates, we identified 51 strains as CPE and divided these into 7 species, 11 E. coli , 14 Klebsiella spp., 15 Enterobacter spp., and 11 others, including 4 additional species. Conjugation experiments via broth mating and using E. coli J53 revealed that 24 isolates can transfer carbapenemase-encoding plasmids. MLST analysis showed that 6 of 11 E. coli isolates belonged to clonal complex 10 (CC10). Of 11 K. pneumoniae strains, 9 unique sequence types (STs) were identified, including ST147. Five types of carbapenemase genes were identified, with the most prevalent being NDM ( n? =?39), which is epidemic in clinical settings in the Philippines. E. coli CC10 and K. pneumoniae ST147, which are often detected in clinical settings, were the dominant strains. In summary, our results indicate that hospital sewage and river water are contaminated by CPE strains belonging to clinically important clonal groups.IMPORTANCE Carbapenemase-producing Enterobacteriaceae (CPE) cause severe health care-associated infections, and their increasing prevalence is a serious concern. Recently, natural ecosystems have been recognized as important reservoirs of antibiotic resistance genes. We investigated the prevalence and genetic characteristics of CPE isolated from the environment (hospital sewage and river water) in the Philippines and found several CPE, including Escherichia coli and other species, with different carbapenemases. The most prevalent carbapenemase gene type was NDM, which is endemic in clinical settings. This study revealed that isolates belonging to carbapenemase-producing E. coli CC10 and K. pneumoniae sequence type 147 (ST147), which are often detected in clinical settings, were dominant in the natural environment. Our work here provides a report on the presence and characteristics of CPE in the environment in the Philippines and demonstrates that both hospital sewage and river water are contaminated by CPE strains belonging to clinically important clonal groups.
机译：本研究旨在评估菲律宾养殖和河流水中肠杆菌肠杆菌（CPE）的患病率和遗传特征，具有典型的热带海洋气候。我们收集了83个医院污水和10河水场所的水样。使用Chromagar Musupercarba鉴定CPE，使用基质辅助激光解吸电离 - 飞行质谱（MALDI-TOF MS）或16S rRNA基因测序鉴定革兰氏阴性菌株。使用PCR和DNA测序鉴定肠杆菌菌株中的抗性基因，并研究了CPE中碳癌酶基因的可转移性与共轭实验。基因分型使用多点序列键入（MLST）进行大肠杆菌和Klebsiella肺炎。在124个肠杆菌中分离物中，我们将51个菌株作为CPE鉴定为CPE，将这些分为7种，11种大肠杆菌，14个Klebsiella SPP。，15个肠杆菌SPP。和11种，包括4种其他物种。通过肉汤配合和使用大肠杆菌J53的共轭实验显示，24分离物可以转移碳碱酶编码的质粒。 MLST分析显示，11e11大肠杆菌分离株属于克隆复合物10（CC10）。 11K.肺炎菌株，9种独特的序列类型（STS），包括ST147。鉴定了五种类型的碳结构酶基因，最普遍的是NDM（n？= 39），这在菲律宾的临床环境中是疫情的。 E. COLI CC10和K.肺炎的ST147经常在临床环境中检测到，是主要的菌株。总之，我们的结果表明，医院污水和河水被属于临床重要克隆基团的CPE菌株污染。产碳碱酶活性的肠杆菌（CPE）引起严重的医疗保健相关感染，并且他们的普遍性越来越严重。最近，自然生态系统被认为是抗生素抗性基因的重要储层。我们调查了菲律宾环境（医院污水和河水）中CPE的患病率和遗传特征，并发现了几种CPE，包括大肠杆菌和其他物种，具有不同的碳结构酶。最普遍的碳结蛋白酶基因类型是NDM，其在临床环境中是特有的。该研究表明，在自然环境中，通常检测到属于氨基丙氨酸酶的E. COLI CC10和K.肺炎素序列型147（ST147）的分离物。我们的工作在这里提供了关于菲律宾环境中CPE的存在和特征的报告，并表明医院污水和河流被属于临床重要克隆群体的CPE菌株污染。

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