• 主题
高级搜索  >
历史搜索 清空历史
首页> 外文期刊>Applied Microbiology >Prevalence and Epidemiology of Non-O157 Escherichia coli Serogroups O26, O103, O111, and O145 and Shiga Toxin Gene Carriage in Scottish Cattle, 2014–2015
【24h】

Prevalence and Epidemiology of Non-O157 Escherichia coli Serogroups O26, O103, O111, and O145 and Shiga Toxin Gene Carriage in Scottish Cattle, 2014–2015

机译：苏格兰牛的非O157大肠杆菌血清血清血清血清小组O26，O103，O111和O145和Shiga毒素基因托运的流行率和流行病学

获取原文
           
页面导航
  • 摘要
  • 著录项
  • 相似文献
  • 相关主题

摘要

Cattle are a reservoir for Shiga toxin-producing Escherichia coli (STEC), zoonotic pathogens that cause serious clinical disease. Scotland has a higher incidence of STEC infection in the human population than the European average. The aim of this study was to investigate the prevalence and epidemiology of non-O157 serogroups O26, O103, O111, and O145 and Shiga toxin gene carriage in Scottish cattle. Fecal samples ( n ?=?2783) were collected from 110 herds in 2014 and 2015 and screened by real-time PCR. Herd-level prevalence (95% confidence interval [CI]) for O103, O26, and O145 was estimated as 0.71 (0.62, 0.79), 0.43 (0.34, 0.52), and 0.23 (0.16, 0.32), respectively. Only two herds were positive for O111. Shiga toxin prevalence was high in both herds and pats, particularly for stx _(2) (herd level: 0.99; 95% CI: 0.94, 1.0). O26 bacterial strains were isolated from 36 herds on culture. Fifteen herds yielded O26 stx -positive isolates that additionally harbored the intimin gene; six of these herds shed highly pathogenic stx _(2)-positive strains. Multiple serogroups were detected in herds and pats, with only 25 herds negative for all serogroups. Despite overlap in detection, regional and seasonal effects were observed. Higher herd prevalence for O26, O103, and stx _(1) occurred in the South West, and this region was significant for stx _(2) at the pat level ( P?=? 0.015). Significant seasonal variation was observed for O145 prevalence, with the highest prevalence in autumn ( P?=? 0.032). Negative herds were associated with Central Scotland and winter. Herds positive for all serogroups were associated with autumn and larger herd size and were not housed at sampling.IMPORTANCE Cattle are reservoirs for Shiga toxin-producing Escherichia coli (STEC), bacteria shed in animal feces. Humans are infected through consumption of contaminated food or water and by direct contact, resulting in serious disease and kidney failure in the most vulnerable. The contribution of non-O157 serogroups to STEC illness was underestimated for many years due to the lack of specific tests. Recently, non-O157 human cases have increased, with O26 STEC of particular note. It is therefore vital to investigate the level and composition of non-O157 in the cattle reservoir and to compare them historically and by the clinical situation. In this study, we found cattle prevalence high for toxin, as well as for O103 and O26 serogroups. Pathogenic O26 STEC were isolated from 14% of study herds, with toxin subtypes similar to those seen in Scottish clinical cases. This study highlights the current risk to public health from non-O157 STEC in Scottish cattle.
机译：牛是滋阴毒素生产大肠杆菌（STEC）的水库，造成严重临床疾病的人群病原体。苏格兰在人口中具有比欧洲平均水平的人口中的STEC感染更高。本研究的目的是探讨苏格兰牛中的非O157血清小组O26，O103，O111和O145和Shiga毒素基因托运的患病率和流行病学。从2014年和2015年的110群中收集粪便样品（N？= 2783），并通过实时PCR筛选。 O103，O26和O145的群体患病率（95％置信区间[CI]）估计为0.71（0.62,0.79），0.43（0.34,0.52）和0.23（0.16,0.32）。 o111只有两个畜群是阳性的。群体和帕斯辛患病率高，特别适用于STX _（2）（畜群：0.99; 95％CI：0.94,1.0）。从36种培养物中分离出O26细菌菌株。十五条群产生O26 STX - 阳性分离物另外留下了内含内基因;这些群中的六个血液均匀致病性STX _（2） - 正性菌株。在畜群和苜蓿中检测到多个血清群，只有25只血清小组的遗产。尽管在检测中重叠，但观察到区域和季节性效果。在西南部发生O26，O103和STX _（1）的牧群流行率较高，该地区对于PAT级别的STX _（2）非常重要（P？= 0.015）。对于O145患病率观察到显着的季节性变化，秋季普及最高（P？= 0.032）。消极群与中央苏格兰和冬季有关。所有血清小组的畜群与秋季和较大的畜群大小有关，并未在抽样中居住。以动物粪便在动物粪便中的细菌脱落的Shiga毒素的大肠杆菌（STEC）的水库。人类通过污染的食物或水消耗和直接接触感染，导致最脆弱的疾病和肾脏衰竭。由于缺乏特定的测试，多年来，非O157血清小组对STEC疾病的贡献被低估了。最近，非O157人类病例增加，特别是O26 STEC。因此，研究牛水库中非O157的水平和组成是至关重要的，并在历史上并通过临床情况进行比较。在这项研究中，我们发现牛流行高毒素，以及O103和O26 Serogroups。致病O26 STEC从14％的研究群中分离出来，毒素亚型类似于苏格兰临床病例中所见的亚型。本研究突出了来自苏格兰牛非O157 STEC的公共健康风险。

著录项

相似文献

  • 外文文献
  • 中文文献
  • 专利
获取原文

客服邮箱：kefu@zhangqiaokeyan.com

京公网安备：11010802029741号 ICP备案号：京ICP备15016152号-6 六维联合信息科技 (北京) 有限公司©版权所有

  • 客服微信

  • 服务号