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Environmental Free-Living Amoebae Can Predate on Diverse Antibiotic-Resistant Human Pathogens

机译：环境自由生活的amoebae可以预测多种抗生素的抗性人类病原体

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Here, we sought to test the resistance of human pathogens to unaltered environmental free-living amoebae. Amoebae are ubiquitous eukaryotic microorganisms and important predators of bacteria. Environmental amoebae have also been proposed to serve as both potential reservoirs and training grounds for human pathogens. However, studies addressing their relationships with human pathogens often rely on a few domesticated amoebae that have been selected to feed on rich medium, thereby possibly overestimating the resistance of pathogens to these predatory phagocytes. From an open-air composting site, we recovered over 100 diverse amoebae that were able to feed on Acinetobacter baumannii and Klebsiella pneumoniae . In a standardized and quantitative assay for predation, the isolated amoebae showed a broad predation spectrum, killing clinical isolates of A. baumannii , K. pneumoniae , Pseudomonas aeruginosa , and Staphylococcus aureus . Interestingly, A. baumannii , which was previously reported to resist predation by laboratory strains of Acanthamoeba , was efficiently consumed by closely related environmental amoebae. The isolated amoebae were capable of feeding on highly virulent carbapenem-resistant or methicillin-resistant clinical isolates. In conclusion, the natural environment is a rich source of amoebae with broad-spectrum bactericidal activities, including against antibiotic-resistant isolates.IMPORTANCE Free-living amoebae have been proposed to play an important role in hosting and disseminating various human pathogens. The resistance of human pathogens to predation by amoebae is often derived from in vitro experiments using model amoebae. Here, we sought to isolate environmental amoebae and to test their predation on diverse human pathogens, with results that challenge conclusions based on model amoebae. We found that the natural environment is a rich source of diverse amoebae with broad-spectrum predatory activities against human pathogens, including highly virulent and antibiotic-resistant clinical isolates.
机译：在这里，我们试图测试人类病原体的抗性，以未经干预的环境自由生物的作用。 Amoebae是普遍存在的真核微生物和细菌的重要捕食者。还提出了环境amoebae作为潜在的储层和人类病原体的培训理由。然而，解决与人类病原体的关系的研究经常依赖于已经选择富含培养基的含有少数驯化的AmoEbae，从而可能估计病原体对这些捕食性吞噬细胞的抗性。从一个露天堆肥网站，我们恢复了100多种不同的Amoebae，能够在鲍曼和克鲁布拉肺炎上喂食。在用于捕获的标准化和定量测定中，分离的作物显示出广泛的捕食光谱，杀死A.Baumannii，K.Pneumoniae，假单胞菌Aeruginosa和金黄色葡萄球菌的临床分离物。有趣的是，A.Baumannii先前据报道抵抗Acanthamoeba的实验室菌株的捕食，通过密切相关的环境Amoebae有效地消耗。孤立的Amoebae能够在高毒性的碳癌耐药或耐甲氧胞蛋白抗性临床分离物上喂养。总之，天然环境是具有广谱杀菌活性的丰富的作物来源，包括抗生素抗性分离物。已经提出了分类的自由生活，在举办和传播各种人类病原体方面发挥重要作用。人类病原体对Amoebae的捕食的抵抗力通常来自使用模型Amoebae的体外实验。在这里，我们寻求孤立环境Amoebae并测试他们对各种人类病原体的捕食，结果挑战基于模型Amoebae的结论。我们发现自然环境是具有广泛的人类病原体的广谱掠夺性活动的丰富普遍活动，包括高毒性和抗生素的临床分离株。

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