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首页> 外文期刊>Applied Microbiology >Pyoverdines Are Essential for the Antibacterial Activity of Pseudomonas chlororaphis YL-1 under Low-Iron Conditions
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Pyoverdines Are Essential for the Antibacterial Activity of Pseudomonas chlororaphis YL-1 under Low-Iron Conditions

机译：纤厚对低铁条件下假单胞菌氯-1的抗菌活性至关重要

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Pseudomonas chlororaphis YL-1 has extensive antimicrobial activities against phytopathogens, and its genome harbors a pyoverdine (PVD) biosynthesis gene cluster. The alternative sigma factor PvdS in Pseudomonas aeruginosa PAO1 acts as a critical regulator in response to iron starvation. The assembly of the PVD backbone starts with peptide synthetase enzyme PvdL. PvdF catalyzes formylation of l-OH-Orn to produce l- N ~(5)-hydroxyornithine. Here, we describe the characterization of PVD production in YL-1 and its antimicrobial activity in comparison with that of its PVD-deficient ΔpvdS , ΔpvdF , and ΔpvdL mutants, which were obtained using a sacB -based site-specific mutagenesis strategy. Using in vitro methods, we examined the effect of exogenous iron under low-iron conditions and an iron-chelating agent under iron-sufficient conditions on PVD production, antibacterial activity, and the relative expression of the PVD transcription factor gene pvdS in YL-1. We found that strain YL-1, the ΔpvdF mutant, and the ΔpvdS (pUCP26- pvdS ) complemented strain produced visible PVDs and demonstrated a wide range of inhibitory effects against Gram-negative and Gram-positive bacteria in vitro under low-iron conditions and that with the increase of iron, its PVD production and antibacterial activity were reduced. The antibacterial compounds produced by strain YL-1 under low-iron conditions were PVDs based on liquid chromatography-tandem mass spectrometry (LC-MS/MS) analysis. Moreover, the antibacterial activity observed in vitro was correlated with in vivo control efficacies of strain YL-1 against rice bacterial leaf blight (BLB) disease caused by Xanthomonas oryzae pv. oryzae. Collectively, PVDs are responsible for the antibacterial activities of strain YL-1 under both natural and induced low-iron conditions.IMPORTANCE The results demonstrated that PVDs are essential for the broad-spectrum antibacterial activities of strain YL-1 against both Gram-positive and Gram-negative bacteria under low-iron conditions. Our findings also highlight the effect of exogenous iron on the production of PVD and the importance of this bacterial product in bacterial interactions. As a biocontrol agent, PVDs can directly inhibit the proliferation of the tested bacteria in addition to participating in iron competition.
机译：Pseudomonas allororaphis Y1-1对植物病变具有广泛的抗微生物活性，其基因组患者是百伏（PVD）生物合成基因簇。铜绿假单胞菌Pao1中的替代Sigma因子PVDS响应于铁饥饿而作为临界调节剂。 PVD骨架的组装从肽合成酶PVD1开始。 PVDF催化L-OH-ORN的甲酰化，以产生L-N〜（5） - 羟基胺。在此，我们描述了与其PVD缺陷型ΔPVDS，ΔPVDF和ΔPVDL突变体的PVD产生的表征及其抗微生物活性，所述PVD缺陷ΔPVDS，ΔPVDF和ΔPVDL突变体使用SacB的位点特异性诱变策略获得。使用体外方法，我们在PVD生产，抗菌活性和PVD转录因子基因PVDS在YL-1中的熨斗上的熨斗中，在低铁条件下和铁螯合剂下的外源铁和铁螯合剂的影响。我们发现菌株YL-1，ΔPVDF突变体和ΔPVDS（PUCP26-PVDS）互补的菌株产生可见的PVD，并在低铁条件下在体外对革兰氏阴性和革兰氏阳性细菌进行广泛的抑制作用随着铁的增加，其PVD产生和抗菌活性降低。在低铁条件下由菌株Y1-1产生的抗菌化合物是基于液相色谱 - 串联质谱（LC-MS / MS）分析的PVDS。此外，在体外观察到的抗菌活性与由Xanthomonas Oryzae PV引起的菌株Y1-1的体内控制效率与水稻细菌叶枯萎病（BLB）病。 oryzae。总的来说，PVDS负责在天然和诱导的低铁条件下的菌株YL-1的抗菌活性。分析结果证明PVDS对于菌株YL-1的广谱抗菌活性对革兰氏阳性和抗菌活性至关重要低铁条件下的革兰氏阴性细菌。我们的研究结果还突出了外源性铁对PVD的生产以及这种细菌产物在细菌相互作用的重要性。作为生物控制剂，除参加铁竞赛外，PVD可直接抑制测试细菌的增殖。

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