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首页> 外文期刊>Applied Microbiology >An Unexpected Role for the Periplasmic Phosphatase PhoN in the Salvage of B6 Vitamers in Salmonella enterica
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An Unexpected Role for the Periplasmic Phosphatase PhoN in the Salvage of B6 Vitamers in Salmonella enterica

机译：在沙门氏菌中B6维生素抢救的周质磷酸酶耳蛋白的意外角色

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摘要

Pyridoxal 5′-phosphate (PLP) is the biologically active form of vitamin B_(6), essential for cellular function in all domains of life. In many organisms, such as Salmonella enterica serovar Typhimurium and Escherichia coli , this cofactor can be synthesized de novo or salvaged from B_(6) vitamers in the environment. Unexpectedly, S. enterica strains blocked in PLP biosynthesis were able to use exogenous PLP and pyridoxine 5′-phosphate (PNP) as the source of this required cofactor, while E. coli strains of the same genotype could not. Transposon mutagenesis found that phoN was essential for the salvage of PLP and PNP under the conditions tested. phoN encodes a class A nonspecific acid phosphatase (EC 3.1.3.2) that is transcriptionally regulated by the PhoPQ two-component system. The periplasmic location of PhoN was essential for PLP and PNP salvage, and in vitro assays confirmed PhoN has phosphatase activity with PLP and PNP as substrates. The data suggest that PhoN dephosphorylates B_(6) vitamers, after which they enter the cytoplasm and are phosphorylated by kinases of the canonical PLP salvage pathway. The connection of phoN with PhoPQ and the broad specificity of the gene product suggest S. enterica is exploiting a moonlighting activity of PhoN for PLP salvage.IMPORTANCE Nutrient salvage is a strategy used by species across domains of life to conserve energy. Many organisms are unable to synthesize all required metabolites de novo and must rely exclusively on salvage. Others supplement de novo synthesis with the ability to salvage. This study identified an unexpected mechanism present in S. enterica that allows salvage of phosphorylated B_(6) vitamers. In vivo and in vitro data herein determined that the periplasmic phosphatase PhoN can facilitate the salvage of PLP and PNP. We suggest a mechanistic working model of PhoN-dependent utilization of PLP and PNP and discuss the general role of promiscuous phosphatases and kinases in organismal fitness.
机译：吡哆醛5'-磷酸盐（PLP）是生物活性的维生素B_（6）的形式，对于所有生命域中的细胞功能必不可少。在许多生物体中，如沙门氏菌肠道血吸虫和大肠杆菌，这种辅助因子可以合成Novo或从环境中的B_（6）维生素中的销售。出乎意料地，在PLP生物合成中封闭的S.肠道菌株能够使用外源PLP和吡哆醇5'-磷酸（PNP）作为这种所需辅因子的来源，而同一基因型的大肠杆菌菌株不能。转座子诱变发现，在测试的条件下，PHP对PLP和PNP的销售至关重要。 Phon编码由PHOPQ双组分系统转录调节的非特异性酸性磷酸酶（EC 3.1.3.2）。 PHP的周质位置对于PLP和PNP校存是必不可少的，并且体外测定证实了PH具有PLP和PNP作为基质的磷酸酶活性。数据表明，Phon Dephosphorylates B_（6）维生素，之后它们进入细胞质，并通过规范PLP抢救途径的激酶磷酸化。使用PHOPQ的响应和基因产物的广泛特异性的接触表达S.Sexica正在利用PLP Salvage的Phonlighting的云峰。分析营养救生是跨生命领域的物种使用的策略来保护能量。许多生物无法合成所有必需的代谢物De Novo，并且必须完全依赖于救助。其他人补充了De Novo合成，具有挽救的能力。该研究确定了S.肠内肠中存在的意外机制，允许磷酸化的B_（6）维生素的校存。在体内和体外数据中，确定周质磷酶的抗性可以促进PLP和PNP的救生。我们建议了PLP和PNP的咽依赖性利用机械工作模型，并讨论了混杂磷酸酶和激酶在有机体健康中的一般作用。

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