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首页> 外文期刊>Applied Microbiology >The Actin Cytoskeleton Mediates Transmission of “Candidatus Liberibacter solanacearum” by the Carrot Psyllid
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The Actin Cytoskeleton Mediates Transmission of “Candidatus Liberibacter solanacearum” by the Carrot Psyllid

机译：肌动蛋白细胞骨架由胡萝卜蚜虫植物介导“Candidatus Libibacter Solanacearum”的传播

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Several vector-borne plant pathogens have evolved mechanisms to exploit and to hijack vector host cellular, molecular, and defense mechanisms for their transmission. In the past few years, Liberibacter species, which are transmitted by several psyllid vectors, have become an economically important group of pathogens that have devastated the citrus industry and caused tremendous losses to many other important crops worldwide. The molecular mechanisms underlying the interactions of Liberibacter species with their psyllid vectors are poorly studied. “ Candidatus Liberibacter solanacearum,” which is associated with important vegetable diseases, is transmitted by the carrot psyllid Bactericera trigonica in a persistent manner. Here, we elucidated the role of the B. trigonica Arp2/3 protein complex, which plays a major role in regulation of the actin cytoskeleton, in the transmission of “ Ca . Liberibacter solanacearum.” “ Ca . Liberibacter solanacearum” colocalized with ArpC2, a key protein in this complex, and this colocalization was strongly associated with actin filaments. Silencing of the psyllid ArpC2 disrupted the colocalization and the dynamics of F-actin. Silencing of RhoGAP21 and Cdc42, which act in the signaling cascade leading to upregulation of Arp2/3 and F-actin bundling, showed similar results. On the other hand, silencing of ArpC5, another component of the complex, did not induce any significant effects on F-actin formation. Finally, ArpC2 silencing caused a 73.4% reduction in “ Ca . Liberibacter solanacearum” transmission by psyllids, strongly suggesting that transmission of “ Ca . Liberibacter solanacearum” by B. trigonica is cytoskeleton dependent and “ Ca . Liberibacter solanacearum” interacts with ArpC2 to exploit the intracellular actin nucleation process for transmission. Targeting this unique interaction could lead to the development of a novel strategy for the management of Liberibacter -associated diseases.IMPORTANCE Plant diseases caused by vector-borne pathogens are responsible for tremendous losses and threaten some of the most important agricultural crops. A good example is the citrus greening disease, which is caused by bacteria of the genus Liberibacter and is transmitted by psyllids; it has devastated the citrus industry in the United States, China, and Brazil. Here, we show that psyllid-transmitted “ Candidatus Liberibacter solanacearum” employs the actin cytoskeleton of psyllid gut cells, specifically the ArpC2 protein in the Arp2/3 complex of this system, for movement and transmission in the vector. Silencing of ArpC2 dramatically influenced the interaction of “ Ca . Liberibacter solanacearum” with the cytoskeleton and decreased the bacterial transmission to plants. This system could be targeted to develop a novel approach for the control of Liberibacter -associated diseases.
机译：几种载体传播的植物病原体已经进化了机制，用于利用和劫持载体宿主细胞，分子和防御机制的变速器。在过去的几年里，由几个脑脂肺粉载体传播的物种已经成为一种经济上重要的病原体，这些病原体摧毁了柑橘行业，并对全世界许多其他重要作物引起了巨大的损失。依赖于其腹股沟媒体载体的物种相互作用的分子机制差异很差。 “Candidatus Libibacter Solanacearum”，与重要植物疾病相关，用胡萝卜囊泡细菌三核，以持续的方式传播。在这里，我们阐明了B.Trigonica ARP2 / 3蛋白复合物的作用，它在“CA的传递中，在肌动蛋白细胞骨架的调节中起主要作用。 Libibacter Solanacearum。“ “加利福尼亚州。 Libisibacter Solanacearum“用Arpc2分致大理，这是该综合体的关键蛋白质，并且这种分致敏感性与肌动蛋白长丝强烈相关。 Psyllid ARPC2的沉默中断了F-Actin的分解化和动态。 rhoGAP21和CDC42的沉默，其在信号传导级联中起作用导致ARP2 / 3和F-Actin捆绑的上调，显示出类似的结果。另一方面，ARPC5的沉默是复合物的另一个组分，对F-actin形成没有诱导任何显着影响。最后，ARPC2沉默引起了“CA”减少了73.4％。 Libibacter Solanacearum“通过囊莲传播，强烈暗示”Ca“的传播。 Libibacter solanacearum“by B. Tregonica是依赖细胞骨架和”Ca. Libibacter Solanacearum“与ARPC2相互作用，利用细胞内肌动蛋白成核法进行透射。针对这种独特的互动可能导致开发一种新的酵母菌疾病管理战略。传染料病原体引起的分析植物疾病负责巨大的损失，威胁到一些最重要的农作物。一个很好的例子是柑橘绿化疾病，这是由单杆菌属的细菌引起的，并且由囊脂糖传播;它在美国，中国和巴西摧毁了柑橘行业。在这里，我们表明，伴随的腹股沟般的“念珠菌单杆菌杆菌”采用肌蛋白细胞骨架，特别是在该系统的ARP2 / 3络合物中的ARPC2蛋白，用于在载体中的运动和透射。 ARPC2的沉默显着影响了“CA”的相互作用。 Libisibacter Solanacearum“具有细胞骨架并降低细菌传播到植物。该系统可以旨在制定一种控制无菌酵母的新方法。

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