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Gene expression and metabolic changes of Momordica charantia L. seedlings in response to low temperature stress

机译：低温胁迫康复幼苗基因表达及果岭幼苗的代谢变化

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Low temperature is one of the abiotic factors limiting germination, growth and distribution of the plant in current plant-products industry, especially for the tropical vegetables in non-tropical area or other fields under cold temperature. Screening the plant with ability against cold temperature captured worldwide attention and exerted great importance. In our previous work, the anti-cold specie of Momordica Charantia L. seedlings was screened out. Yet, the molecular and physiological mechanisms underlying this adaptive process still remain unknown. This study was aimed to investigate adaption mechanism of anti-cold species of Momordica Charantia L. seedlings in genetical and metabolomics levels. Two species, cold-susceptible group (Y17) and cold-resistant group (Y54), were evaluated containing the indexes of malondialdehyde (MDA), hydrogen peroxide (H 2 O 2 ), proline content, activities of antioxidant enzymes, metabolites changes and genes differentiation in plant tissues after cold treatment. It found that low temperature stress resulted in increased accumulation of MDA, H 2 O 2 and proline content in two species, but less expressions in cold-resistant species Y54. As compared to Y17, cold-resistant species Y54 presented significantly enhanced antioxidant enzyme activities of POD (peroxidase), CAT (cataalase) and SOD (superoxide dismutase). Meanwhile, higher expressed genes encoded antioxidant enzymes and transcription factors when exposure to the low temperature were found in cold-resistant species Y54, and core genes were explored by Q-PCR validation, including McSOD1, McPDC1 and McCHS1. Moreover, plant metabolites containing amino acid, sugar, fatty acid and organic acid in Y54 were higher than Y17, indicating their important roles in cold acclimation. Meanwhile, initial metabolites, including amimo acids, polypeptides, sugars, organic acids and nucleobases, were apparently increased in cold resistant species Y54 than cold susceptible species Y17. Our results demonstrated that the Momordica Charantia L. seedlings achieved cold tolerance might be went through mobilization of antioxidant systems, adjustment of the transcription factors and accumulation of osmoregulation substance. This work presented meaning information for revealing the anti-cold mechanism of the Momordica Charantia L. seedlings and newsight for further screening of anti-cold species in other plant.
机译：低温是限制当前植物产品行业植物萌发，生长和分布的非生物因子之一，特别是对于在寒冷温度下的非热带区域或其他领域的热带蔬菜。筛选植物的抗寒温度能够捕获全球关注并具有重要的重视。在我们以前的工作中，筛选出苦瓜的抗寒冷物种幼苗。然而，这种自适应过程的分子和生理机制仍然是未知的。本研究旨在调查苦难和代谢组水平的苦瓜幼苗抗寒物种的适应机制。评价两个物种，冷易感组（Y17）和抗抗性组（Y54），含有丙二醛（MDA），过氧化氢（H 2 O 2），脯氨酸含量，抗氧化酶活性，代谢物变化和变化冷治疗后植物组织中的基因分化。发现低温胁迫导致MDA，H 2 O 2和脯氨酸含量增加了两种物种，但在抗抗抗性物种Y54中表达较低。与Y17相比，抗抗性物种Y54呈现显着增强的荚（过氧化物酶），猫（二氧化酶）和SOD（超氧化物歧化酶）的抗氧化酶活性。同时，在抗抗性物种Y54中发现暴露于低温时，较高表达基因编码抗氧化酶和转录因子，并且通过Q-PCR验证探索核心基因，包括MCSOD1，MCPDC1和MCCHS1。此外，含有氨基酸，糖，脂肪酸和Y54的植物代谢物高于Y17，表明它们在冷驯化中的重要作用。同时，初始代谢物，包括氨酸，多肽，糖，有机酸和核碱基，显然在抗抗性物种Y54中显然增加，而不是冷易感物种Y17。我们的研究结果表明，苦瓜菌幼苗实现了抗氧化系统的耐寒性，调整转录因子和Osmoreculation物质的积累。这项工作提出了揭示苦瓜幼苗抗寒机制的意义信息，并对其他植物进一步筛查抗寒物种的新闻。

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