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首页> 外文期刊>PLoS One >Microcultivation and FTIR spectroscopy-based screening revealed a nutrient-induced co-production of high-value metabolites in oleaginous Mucoromycota fungi
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Microcultivation and FTIR spectroscopy-based screening revealed a nutrient-induced co-production of high-value metabolites in oleaginous Mucoromycota fungi

机译：基于微流量和基于FTIR光谱的筛选揭示了含有植物丧失的高价代谢物的营养诱导的含有蛋白质霉菌的真菌

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Mucoromycota fungi possess a versatile metabolism and can utilize various substrates for production of industrially important products, such as lipids, chitin/chitosan, polyphosphates, pigments, alcohols and organic acids. However, as far as commercialisation is concerned, establishing industrial biotechnological processes based on Mucoromycota fungi is still challenging due to the high production costs compared to the final product value. Therefore, the development of co-production concept is highly desired since more than one valuable product could be produced at the time and the process has a potentially higher viability. To develop such biotechnological strategy, we applied a high throughput approach consisting of micro-titre cultivation and FTIR spectroscopy. This approach allows single-step biochemical fingerprinting of either fungal biomass or growth media without tedious extraction of metabolites. The influence of two types of nitrogen sources and different levels of inorganic phosphorus on the co-production of lipids, chitin/chitosan and polyphosphates for nine different oleaginous Mucoromycota fungi was evaluated. FTIR analysis of biochemical composition of Mucoromycota fungi and biomass yield showed that variation in inorganic phosphorus had higher effect when inorganic nitrogen source–ammonium sulphate–was used. It was observed that: (1) Umbelopsis vinacea reached almost double biomass yield compared to other strains when yeast extract was used as nitrogen source while phosphorus limitation had little effect on the biomass yield; (2) Mucor circinelloides , Rhizopus stolonifer , Amylomyces rouxii , Absidia glauca and Lichtheimia corymbifera overproduced chitin/chitosan under the low pH caused by the limitation of inorganic phosphorus; (3) Mucor circinelloides , Amylomyces rouxii , Rhizopus stolonifer and Absidia glauca were able to store polyphosphates in addition to lipids when high concentration of inorganic phosphorus was used; (4) the biomass and lipid yield of high-value lipid producers Mortierella alpina and Mortierella hyalina were significantly increased when high concentrations of inorganic phosphorus were combined with ammonium sulphate, while the same amount of inorganic phosphorus combined with yeast extract showed negative impact on the growth and lipid accumulation. FTIR spectroscopy revealed the co-production potential of several oleaginous Mucoromycota fungi forming lipids, chitin/chitosan and polyphosphates in a single cultivation process.
机译：粘性霉菌真菌具有多功能的新陈代谢，可以利用各种基材，用于生产工业上重要的产品，如脂质，几丁质/壳聚糖，多磷酸盐，颜料，醇和有机酸。然而，就商业化而言，由于与最终产品价值相比，由于高生产成本，建立基于粘液霉菌真菌的工业生物技术过程仍然具有挑战性。因此，由于当时可以生产多于一个有价值的产品，因此可以产生多于一个有价值的产品，并且该过程具有潜在较高的存活率，因此非常需要开发。为了制定这种生物技术策略，我们应用了一种高滴度培养和FTIR光谱的高通量方法。这种方法允许无菌生物量或生长培养基的单步生化指纹识别，而不会繁琐地提取代谢物。评估了两种类型的氮源和不同水平的无机磷对脂质，几丁质/壳聚糖和九种不同含粘膜霉菌的共磷酸盐的共同产生的影响。粘性霉菌生物化学组成的FTIR分析和生物质产量的生化组合物表明，当使用无机氮源 - 硫酸铵 - 硫酸铵 - 硫酸铵 - 硫酸铵 - 硫酸铵磷的变化具有更高的效果。观察到：（1）当酵母提取物用作氮源时，与其他菌株相比，umbelopsis vinacea与其他菌株相比达到几乎是双重的生物量产量，而磷局限性对生物质产量没有影响; （2）MUCOR环素，Rhizopus Stolonifer，氨基胺Rouxii，Absidia glauca和Lichtheimia Corymbifera过量的几丁质/壳聚糖在无机磷的限制引起的低pH下; （3）粘膜循环素，淀粉胺rouxii，rhizopus stolonifer和斑氨酰胺能够在使用高浓度的无机磷时除脂质外还能够储存多磷酸盐; （4）当高浓度的无机磷与硫酸铵合并时，高价值脂质生产者的生物量和脂质产量Martierella Alpina和Mortierella凯丽那缘显着增加，而相同量的与酵母提取物联合的无机磷量对此产生负面影响生长和脂质积累。 FTIR光谱揭示了在单一培养过程中形成脂质，几丁质/壳聚糖和多磷酸盐的几种含油粘膜霉菌真菌的共产生潜力。

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