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首页> 外文期刊>Journal of bacteriology >AbaM Regulates Quorum Sensing, Biofilm Formation, and Virulence in Acinetobacter baumannii
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AbaM Regulates Quorum Sensing, Biofilm Formation, and Virulence in Acinetobacter baumannii

机译：ABAM调节法定感测，生物膜形成和血管腺素植物的毒力

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摘要

Acinetobacter baumannii possesses a single divergent luxR / luxRI -type quorum-sensing (QS) locus named abaR / abaI . This locus also contains a third gene located between abaR and abaI , which we term abaM , that codes for an uncharacterized member of the RsaM protein family known to regulate N -acylhomoserine lactone (AHL)-dependent QS in other beta- and gammaproteobacteria. Here, we show that disruption of abaM via a T26 insertion in A. baumannii strain AB5075 resulted in increased production of N -(3-hydroxydodecanoyl)-l-homoserine lactone and enhanced surface motility and biofilm formation. In contrast to the wild type and the abaI ::T26 mutant, the virulence of the abaM ::T26 mutant was completely attenuated in a Galleria mellonella infection model. Transcriptomic analysis of the abaM ::T26 mutant revealed that AbaM differentially regulates at least 76 genes, including the csu pilus operon and the acinetin 505 lipopeptide biosynthetic operon, that are involved in surface adherence, biofilm formation and virulence. A comparison of the wild type, abaM ::T26 and abaI ::T26 transcriptomes, indicates that AbaM regulates ～21% of the QS regulon including the csu operon. Moreover, the QS genes ( abaI and abaR ) were among the most upregulated in the abaM ::T26 mutant. A. baumannii lux -based abaM reporter gene fusions revealed that abaM expression is positively regulated by QS but negatively autoregulated. Overall, the data presented in this work demonstrates that AbaM plays a central role in regulating A. baumannii QS, virulence, surface motility, and biofilm formation. IMPORTANCE Acinetobacter baumannii is a multiantibiotic-resistant pathogen of global health care importance. Understanding Acinetobacter virulence gene regulation could aid the development of novel anti-infective strategies. In A. baumannii, the abaR and abaI genes that code for the receptor and synthase components of an N -acylhomoserine (AHL) lactone-dependent quorum sensing system (QS) are separated by abaM . Here, we show that although mutation of abaM increased AHL production, surface motility, and biofilm development, it resulted in the attenuation of virulence. AbaM was found to control both QS-dependent and QS-independent genes. The significance of this work lies in the identification of AbaM, an RsaM ortholog known to control virulence in plant pathogens, as a modulator of virulence in a human pathogen.
机译：AcineTobacter Baumannii拥有一个不同的Luxr / Luxri -Type Quorum-Sense（QS）基因座，名为Apar / Abai。该基因座还含有位于ABAR和ABAI之间的第三基因，我们在ABAM术语中，该核对已知的RSAM蛋白家族的非特征成员的代码在其他β-和γ-和γ-和γ-和γ-和γ-和γ-癌菌中调节N-acylhomoserine内酯（AHL）依赖性Qs。这里，我们表明，通过T26插入A ABAM的破坏.Baumannii菌株AB5075导致生产的N - （3-羟基二癸酰）-L-甲晶内酯和增强的表面运动和生物膜形成产生。与野生型和ABAI :: T26突变体形成鲜明比，ABAM :: T26突变体的毒力完全衰减在Galleria Mellonella感染模型中。 ABAM :: T26突变体的转录组分析显示，ABAM差异地调节至少76个基因，包括CSU Pilus操纵子和脂素505脂肽生物合成型型型型型型型型型型型型型型型型型型型型型型型型型型型型型型型型型型型型型型型型型型型型型型型型型型型型型型型型型型型型型型型型型型型型型型型型型型型型术语包括表面粘附，生物膜形成和毒力。野生类型，ABAM :: T26和ABAI :: T26转录om的比较表明ABAM调节〜21％的QS QS调节件，包括CSU操纵子。此外，QS基因（ABAI和ABAR）是ABAM :: T26突变体中最上调的。 A. Baumannii Lux - 基础的ABAM报告者基因融合揭示了ABAM表达被QS呈正常调节，而是对QS呈正常调节。总的来说，本工作中提出的数据表明，ABAM在调节A.Baumannii QS，毒力，表面运动和生物膜形成方面发挥着核心作用。 Paumannii的重要性是全球医疗保健重要性的一种多纤维病病原体。了解毒致毒力基因调节可以帮助开发新的抗感染策略。在A.Baumannii中，ABAR和ABAI基因代码N-acylhomoserine（AHL）内酯依赖于Quorum传感系统（QS）的受体和合成酶组分代码通过ABAM分离。在这里，我们表明，尽管ABAM的突变增加了AHL的生产，表面运动和生物膜发育，导致毒力的衰减。发现ABAM控制QS依赖性和QS的基因。这项工作的重要性在于鉴定ABAM，熟悉植物病原体中的毒力的RSAM正直，作为人病原体中的毒力调节剂。

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