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首页> 外文期刊>Journal of bacteriology >Pilus Production in Acinetobacter baumannii Is Growth Phase Dependent and Essential for Natural Transformation
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Pilus Production in Acinetobacter baumannii Is Growth Phase Dependent and Essential for Natural Transformation

机译：血管发射菌的菌毛产量是生长期依赖性，对自然转型至关重要

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Acinetobacter baumannii is a severe threat to human health as a frequently multidrug-resistant hospital-acquired pathogen. Part of the danger from this bacterium comes from its genome plasticity and ability to evolve quickly by taking up and recombining external DNA into its own genome in a process called natural competence for transformation. This mode of horizontal gene transfer is one of the major ways that bacteria can acquire new antimicrobial resistances and toxic traits. Because these processes in A. baumannii are not well studied, we herein characterized new aspects of natural transformability in this species that include the species’ competence window. We uncovered a strong correlation with a growth phase-dependent synthesis of a type IV pilus (TFP), which constitutes the central part of competence-induced DNA uptake machinery. We used bacterial genetics and microscopy to demonstrate that the TFP is essential for the natural transformability and surface motility of A. baumannii, whereas pilus-unrelated proteins of the DNA uptake complex do not affect the motility phenotype. Furthermore, TFP biogenesis and assembly is subject to input from two regulatory systems that are homologous to Pseudomonas aeruginosa, namely, the PilSR two-component system and the Pil-Chp chemosensory system. We demonstrated that these systems affect not only the piliation status of cells but also their ability to take up DNA for transformation. Importantly, we report on discrepancies between TFP biogenesis and natural transformability within the same genus by comparing data for our work on A. baumannii to data reported for Acinetobacter baylyi, the latter of which served for decades as a model for natural competence. IMPORTANCE Rapid bacterial evolution has alarming negative impacts on animal and human health which can occur when pathogens acquire antimicrobial resistance traits. As a major cause of antibiotic-resistant opportunistic infections, A. baumannii is a high-priority health threat which has motivated renewed interest in studying how this pathogen acquires new, dangerous traits. In this study, we deciphered a specific time window in which these bacteria can acquire new DNA and correlated that with its ability to produce the external appendages that contribute to the DNA acquisition process. These cell appendages function doubly for motility on surfaces and for DNA uptake. Collectively, we showed that A. baumannii is similar in its TFP production to Pseudomonas aeruginosa, though it differs from the well-studied species A. baylyi.
机译：AcineTobacter Baumannii是对人类健康的严重威胁，作为常见的抗性医院获得的病原体。来自这种细菌的一部分危险来自其基因组可塑性，并通过在称为自然能力转化的过程中进入其自身的基因组来快速发展的能力。这种水平基因转移模式是细菌可以获得新的抗微生物抗性和有毒状性状的主要方法之一。因为A.Baumannii中的这些过程没有很好地研究，所以我们在此表征了该物种中的自然变革性的新方面，包括物种能力窗口。我们发现与IV型菌落（TFP）的生长相位依赖性合成的强烈相关，其构成了能力诱导的DNA吸收机构的中心部分。我们使用细菌遗传学和显微镜证明TFP对于A.Baumannii的天然变革性和表面活力至关重要，而DNA摄取复合物的菌毛无关蛋白质不会影响运动表型。此外，TFP生物发生和组装受到两种调节系统的输入，其与假单胞菌铜绿假单胞菌同源，即Pilsr双组分系统和Pil-CHP化学感受系统。我们证明这些系统不仅影响细胞的突破状态，而且影响其占用DNA进行转化的能力。重要的是，我们通过比较我们对A.Baumannii的数据进行比较对百叶腺素的数据来报告的数据，报告了同一属的TFP生物发生和自然变革性的差异。重要性快速细菌演化对动物和人类健康产生了惊人的负面影响，当病原体获得抗微生物抗性性状时可能发生。作为抗生素抗性机会感染的主要原因，A.Baumannii是一种高优先的健康威胁，该威胁已经激励了研究该病原体如何获得新的危险性状。在这项研究中，我们破译了一种特定的时间窗口，其中这些细菌可以获取新的DNA并与其产生有助于DNA获取过程的外部附属物的能力。这些细胞阑尾函数在表面上的运动和DNA摄取的函数倍增。总的来说，我们认为A.Baumannii在其TFP产量中与铜绿假单胞院相似，尽管它与学习的种类A.百叶窗不同。

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