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首页> 外文期刊>BMC Infectious Diseases >Molecular epidemiology and genetic diversity of norovirus among hospitalized children with acute gastroenteritis in Tianjin, China, 2018–2020
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Molecular epidemiology and genetic diversity of norovirus among hospitalized children with acute gastroenteritis in Tianjin, China, 2018–2020

机译：天津急性胃肠炎住院儿童中诺病毒的分子流行病学和遗传多样性，2018 - 2012

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摘要

Norovirus (NoV) is a major cause of viral acute gastroenteritis (AGE) in children worldwide. Epidemiological analysis with respect to the virus strains is limited in China. This study aimed to investigate the prevalence, patterns, and molecular characteristics of NoV infection among children with AGE in China. A total 4848 stool samples were collected from children who were admitted with AGE in Tianjin Children’s Hospital from August 2018 to July 2020. NoV was preliminarily detected using real-time reverse transcription polymerase chain reaction (RT-PCR). Partial sequences of the RNA-dependent RNA polymerase (RdRp) and capsid genes of positive samples were amplified by conventional RT-PCR and then sequenced. The NoV genotype was determined by online Norovirus Typing Tool Version 2.0, and phylogenetic analysis was conducted using MEGA 6.0. The prevalence of NoV was 26.4% (1280/4848). NoV was detected in all age groups, with the 7–12?months group having the highest detection rate (655/2014, 32.5%). NoV was detected during most part of the year with higher frequency in winter than other seasons. Based on the genetic analysis of RdRp, GII. Pe was the most predominant genotype detected at 70.7% (381/539) followed by GII.P12 at 25.4% (137/539). GII.4 was the most predominant capsid genotype detected at 65.3% (338/518) followed by GII.3 at 26.8% (139/518). Based on the genetic analysis of RdRp and capsid sequences, the strains were clustered into 10 RdRp–capsid genotypes: GII.Pe-GII.4 Sydney 2012 (65.5%), GII.P12-GII.3 (27.2%), GII.P16-GII.2 (1.8%), GII.P12-GII.2 (0.2%), GII.P17-GII.17 (1.1%), GII.Pe-GII.3 (1.8%), GII.Pe-GII.2 (1.1%), GII.Pe-GII.1 (0.4%), GII.16-GII.4 Sydney 2012 (0.7%), and GII.P7-GII.6 (0.2%). The predominant NoV genotypes changed from GII.Pe-GII.4 Sydney 2012 and GII.P12-GII.3 between August 2018 and July 2019 to GII.Pe-GII.4 Sydney 2012 and GII.P16-GII.2 between August 2019 and July 2020. The patients with GII.Pe-GII.4 Sydney 2012 genotype were more likely to suffer from vomiting symptom than those with GII.P12-GII.3. NoV is an important pathogen responsible for viral AGE among children in China. GII.Pe-GII.4 Sydney 2012 and GII.P12-GII.3 were major recombinant genotypes. Knowledge of circulating genotypes and seasonal trends is of great importance for disease prevention and surveillance.
机译：诺罗病毒（11月）是全世界儿童病毒急性胃肠炎（年龄）的主要原因。关于病毒菌株的流行病学分析在中国有限。本研究旨在探讨中国年龄增长的儿童11人口感染的患病率，模式和分子特征。从2018年8月至2020年7月，从天津儿童医院预订的儿童收集了4848凳样本。使用实时逆转录聚合酶链反应（RT-PCR）预先检测到11月。通过常规的RT-PCR扩增RNA依赖性RNA聚合酶（RDRP）和胶囊基因的偏见序列，然后测序。通过在线诺维病毒键入工具3.0版测定的11个基因型，并且使用Mega 6.0进行系统发育分析。 NOV的患病率为26.4％（1280/4848）。 11月在所有年龄组中检测到，7-12？几个月组，检测率最高（655 / 2014,32.5％）。在今年大部分时间内检测到11月，比其他季节更高的频率。基于RDRP，GII的遗传分析。 PE是在70.7％（381/539）中检测到的最主要的基因型，然后在25.4％（137/539）。 GII.4是65.3％（338/518）检测到的最主要胶囊基因型，然后在26.8％（139/518）。基于RDRP和衣壳序列的遗传分析，将菌株聚集成10个RDRP-Capsid基因型：GII.PE-GII.4悉尼2012（65.5％），GII.P12-GII.3（27.2％），GII。 P16-GII.2（1.8％），GII.P12-GII.2（0.2％），GII.P17-GII.17（1.1％），GII.PE-GII.3（1.8％），GII.PE- GII.2（1.1％），GII.PE-GII.1（0.4％），GII.16-GII.4悉尼2012（0.7％）和GII.P7-GII.6（0.2％）。主要的11月基因型从GII.PE-GII.4悉尼2012年和2019年8月至2019年7月至2012年八月至2012年之间发生变为GII.PE-GII.3和7月2020日。患有GII.PE-GII.4悉尼2012年基因型的患者更容易患有呕吐症状，而不是GII.P12-GII.3。 11月是对中国儿童中病毒年龄负责的重要病原体。 GII.PE-GII.4悉尼2012年和GII.P12-GII.3是主要的重组基因型。循环基因型和季节性趋势的知识对于疾病预防和监测非常重要。

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