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首页> 外文期刊>BMC Microbiology >Analysis of virulence phenotypes and antibiotic resistance in clinical strains of Acinetobacter baumannii isolated in Nashville, Tennessee
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Analysis of virulence phenotypes and antibiotic resistance in clinical strains of Acinetobacter baumannii isolated in Nashville, Tennessee

机译：田纳西州纳什维尔分离植物临床菌株毒力表型及抗生素抗性分析

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Acinetobacter baumannii is a gram-negative bacterium which causes opportunistic infections in immunocompromised hosts. Genome plasticity has given rise to a wide range of strain variation with respect to antimicrobial resistance profiles and expression of virulence factors which lead to altered phenotypes associated with pathogenesis. The purpose of this study was to analyze clinical strains of A. baumannii for phenotypic variation that might correlate with virulence phenotypes, antimicrobial resistance patterns, or strain isolation source. We hypothesized that individual strain virulence phenotypes might be associated with anatomical site of isolation or alterations in susceptibility to antimicrobial interventions. A cohort of 17 clinical isolates of A. baumannii isolated from diverse anatomical sites were evaluated to ascertain phenotypic patterns including biofilm formation, hemolysis, motility, and antimicrobial resistance. Antibiotic susceptibility/resistance to ampicillin-sulbactam, amikacin, ceftriaxone, ceftazidime, cefotaxime, ciprofloxacin, cefepime, gentamicin, levofloxacin, meropenem, piperacillin, trimethoprim-sulfamethoxazole, ticarcillin- K clavulanate, tetracyclin, and tobramycin was determined. Antibiotic resistance was prevalent in many strains including resistance to ampicillin-sulbactam, amikacin, ceftriaxone, ceftazidime, cefotaxime, ciprofloxacin, cefepime, gentamicin, levofloxacin, meropenem, piperacillin, trimethoprim-sulfamethoxazole, ticarcillin- K clavulanate, tetracyclin, and tobramycin. All strains tested induced hemolysis on agar plate detection assays. Wound-isolated strains of A. baumannii exhibited higher motility than strains isolated from blood, urine or Foley catheter, or sputum/bronchial wash. A. baumannii strains isolated from patient blood samples formed significantly more biofilm than isolates from wounds, sputum or bronchial wash samples. An inverse relationship between motility and biofilm formation was observed in the cohort of 17 clinical isolates of A. baumannii tested in this study. Motility was also inversely correlated with induction of hemolysis. An inverse correlation was observed between hemolysis and resistance to ticarcillin-k clavulanate, meropenem, and piperacillin. An inverse correlation was also observed between motility and resistance to ampicillin-sulbactam, ceftriaxone, ceftoxamine, ceftazidime, ciprofloxacin, or levofloxacin. Strain dependent variations in biofilm and motility are associated with anatomical site of isolation. Biofilm and hemolysis production both have an inverse association with motility in the cohort of strains utilized in this study, and motility and hemolysis were inversely correlated with resistance to numerous antibiotics.
机译：AcineTobacter Baumannii是一种革兰氏阴性细菌，导致免疫染色体中的机会性感染。基因组可塑性具有相对于抗微生物抗性分布的广泛的应变变化和毒力因子的表达，导致与发病机制相关的改变表型。本研究的目的是分析A.Baumannii的临床菌株，用于表型变异，可能与毒力表型，抗微生物抗性图案或应变隔离源相关。我们假设个体应变毒力表型可能与抗菌干预易感性的分离或改变的解剖部位相关。评估从不同解剖部位分离的A.Baumannii的17个临床分离株的群组，以确定表型模式，包括生物膜形成，溶血，运动和抗微生物抗性。测定了抗生素易感性/抗氨苄青霉素 - 抑制症，Amikacin，头孢菌蛋白，头孢唑胺，头孢噻肟，庆大霉素，左氧氟沙星，梅洛芬，哌啶素，三甲基磺酰甲恶唑，TiCarcillin-K Clavulanate，四环素和染发蛋白。许多菌株中抗生素抗性普遍存在，包括抗氨苄青霉素 - 苏酰胺，氨基嘧啶，头孢菌，头孢他啶，头孢噻肟，环丙沙星，头孢噻肟，庆大霉素，左氧氟沙星，梅洛涅姆，哌啶素，三甲双胍，TiCarcillin-K Clavulanate，四环素和染发蛋白。所有菌株测试诱导琼脂平板检测测定溶血。伤口分离的A.Baumannii表现出比血液，尿液或粪便导管分离的菌株更高的运动，或痰/支气管洗涤。 A.从患者血液样本中分离的Baumannii菌株比伤口，痰或支气管洗涤样品的分离物形成明显更多的生物膜。在本研究中A.Baumannii的17个临床分离株的群组中观察到运动和生物膜形成的反比关系。运动性也与溶血性同时相关。在溶血性和抗替纳米霉素-K Clavulanate，Meropenem和Piperacillin之间观察到逆相关性。在对氨苄青霉素 - 抑菌，头孢哌酮，Ceftoxamine，Ceftazidime，Ciprofloxac或左氧氟沙星的运动性和抗性之间也观察到反向相关性。生物膜的应变依赖性变化和运动与分离的解剖部位相关。生物膜和溶血产量均具有与本研究中使用的菌株队列中的运动性反比转向，并且运动性和溶血与抗性与无数抗生素的抗性相反。

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