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首页> 外文期刊>Allergy, Asthma & Clinical Immunology >Expression levels of plasma exosomal miR-124, miR-125b, miR-133b, miR-130a and miR-125b-1-3p in severe asthma patients and normal individuals with emphasis on inflammatory factors
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Expression levels of plasma exosomal miR-124, miR-125b, miR-133b, miR-130a and miR-125b-1-3p in severe asthma patients and normal individuals with emphasis on inflammatory factors

机译：血浆外泌体miR-124，miR-125b，miR-133b，miR-130a和miR-125b-1-3p在严重哮喘患者和正常个体上强调炎症因子的表达水平

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Identification of molecular markers, such as miRNAs is promising for the diagnosis of asthma and its clinical phenotypes. The aim of this study was to examine the changes in the expression of selected microRNAs in plasma exosomal fractions of severe asthma patients. The expression of miRNAs was determined in relation to the changes in inflammatory markers. Severe asthma patients (n?=?30) and healthy subjects (n?=?30) were selected among the individuals referred to asthma and allergy clinic. Blood was collected from each participant to determine the serum high-sensitive C-reactive protein (hs-CRP) and total IgE. The exosomal fraction of plasma was isolated and processed for quantitation of miR-124, miR-125b, miR-133b, miR-130a and miR-125b-1-3p expression using quantitative real time-PCR (qRT-PCR). Serum hs-CRP and total IgE were significantly higher in asthma patients compared to controls. Expression of miR-124, miR-133b, and miR-130a was down-regulated in asthma patients as compared to controls (p??0.0001). However, the expression of miR-125b was substantially higher in patients compared to controls (p??0.0001). There was no significant difference in the expression of miR-125b-1-3p in the patients and controls. Data analysis revealed that among the miRNAs, changes in miR-125b in severe asthma patients were highly correlated with the serum levels of hs-CRP and IgE. Overexpression of miR-125b in severe asthma which was associated with serum IgE and hs-CRP may suggest that this molecule is linked to inflammatory reactions. Up-regulation of miR-125b together with decreased expression of miR-124, miR-133b, and miR-130a may suggest that this miRNA profile is useful for diagnosis and discrimination of clinical phenotypes of asthma.
机译：鉴定分子标记，例如miRNA是对哮喘诊断的诊断及其临床表型。本研究的目的是检查严重哮喘患者的血浆外泌体部分中所选MicroRNA表达的变化。确定miRNA的表达与炎症标志物的变化有关。在引用哮喘和过敏诊所的个体中选择严重的哮喘患者（N？= 30）和健康受试者（N？=？30）。从每个参与者收集血液以确定血清高敏感的C-反应蛋白（HS-CRP）和总IgE。分离血浆的外泌体级分，并使用定量实时-PCR（QRT-PCR）定量miR-124，miR-125b，miR-133b，miR-130a和miR-125b-1-3p表达。与对照相比，哮喘患者血清HS-CRP和总IgE显着高。与对照组相比然而，与对照相比，患者MiR-125B的表达在患者中基本上更高（P？β01.01）。患者和对照组miR-125b-1-3p的表达没有显着差异。数据分析显示，在MIRNA中，严重哮喘患者MIR-125B的变化与HS-CRP和IgE的血清水平高度相关。与血清IgE和HS-CRP相关的严重哮喘中miR-125b的过表达可能表明该分子与炎症反应相关。 miR-125b的上调与miR-124，miR-133b和miR-130a的降低可能表明该miRNA型材可用于诊断和辨别哮喘的临床表型。

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