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首页> 外文期刊>Artificial cells, nanomedicine, and biotechnology. >Targeting regulation of the tumour microenvironment induces apoptosis of breast cancer cells by an affinity hemoperfusion adsorbent
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Targeting regulation of the tumour microenvironment induces apoptosis of breast cancer cells by an affinity hemoperfusion adsorbent

机译：肿瘤微环境的靶向调节通过亲和血液灌注吸附剂诱导乳腺癌细胞的凋亡

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The cytokine network of tumour microenvironment (TME) plays an important role in cancer growth and progression. The current work aims to provide a new strategy for cancer therapy based on the targeted regulation of cytokines in the TME. Here, heparin-coupled polyvinyl alcohol (PVA-H) microspheres have been developed as an adsorbent for selectively remove tumour-induced immunosuppressive cytokines, such as vascular endothelial growth factor (VEGF) and transforming growth factor-beta (TGF-β), but not tumour necrosis factor-alpha (TNF-α) which has an immune-stimulating effect and can inhibit tumour growth. The proliferation and apoptosis of breast cancer cells after perfusion were tested by cell viability assays, flow cytometry analysis and mRNA microarray assays. Results showed that the PVA-H microspheres efficiently absorbed the majority of VEGF (74.39%) and TGF-β (86.39%), but much less TNF-α (4.16%). The regulation of the cytokines had remarkable anti-proliferative and pro-apoptotic effects on breast cancer cells, which was further confirmed from the change of mRNA expression levels. Thus, targeting regulatory pathways within the TME by an affinity adsorbent that selectively depletes immunosuppressive cytokines is potentially a new and promising strategy for cancer therapy.
机译：肿瘤微环境（TME）的细胞因子网络在癌症生长和进展中起着重要作用。目前的工作旨在基于TME中的细胞因子的靶向调节提供一种新的癌症治疗策略。这里，已经开发了肝素偶联的聚乙烯醇（PVA-H）微球作为吸附剂，用于选择性地去除肿瘤诱导的免疫抑制细胞因子，例如血管内皮生长因子（VEGF）和转化生长因子-β（TGF-β），但是不是具有免疫刺激作用的肿瘤坏死因子-α（TNF-α）并且可以抑制肿瘤生长。通过细胞活力测定，流式细胞术分析和mRNA微阵列测定测试灌注后乳腺癌细胞的增殖和凋亡。结果表明，PVA-H微球有效地吸收了大部分VEGF（74.39％）和TGF-β（86.39％），但TNF-α（4.16％）。细胞因子的调节对乳腺癌细胞具有显着的抗增殖和促凋亡作用，从mRNA表达水平的变化进一步证实了这一点。因此，通过亲和力吸附剂在TME内靶向调节途径，可选择性地耗尽免疫抑制细胞因子是癌症治疗的一种新的和有希望的策略。

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