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首页> 外文期刊>Influenza and other respiratory viruses. >Cepheid Xpert ? Flu/RSV and Seegene Allplex ? RP1 show high diagnostic agreement for the detection of influenza A/B and respiratory syncytial viruses in clinical practice
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Cepheid Xpert ? Flu/RSV and Seegene Allplex ? RP1 show high diagnostic agreement for the detection of influenza A/B and respiratory syncytial viruses in clinical practice

机译：cepheid xpert？ 流感/ RSV和Seegene Allplex？ RP1显示出高诊断协议，用于检测临床实践中的流感A / B和呼吸道合胞病毒

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BACKGROUND:Molecular assays based on reverse transcription-polymerase chain reaction (RT-PCR) provide reliable results for the detection of respiratory pathogens, although diagnostic agreement varies. This study determined the agreement between the RT-PCR assays (Xpert ? Flu/RSV vs Allplex ? RP1) in detecting influenza A, influenza B, and respiratory syncytial viruses (RSVs) in clinical practice.METHODS:We retrospectively identified 914 patient encounters where testing with both Xpert ? Flu/RSV and Allplex ? RP1 was undertaken between October 2015 and September 2019 in seven hospitals across New South Wales, Australia. The diagnostic agreement of the two assays was evaluated using positive percent agreement, negative percent agreement, and prevalence and bias-adjusted kappa.RESULTS:The positive percent agreement was 95.1% for influenza A, 87.5% for influenza B, and 77.8% for RSV. The negative percent agreement was 99.4% for influenza A, 99.9% for influenza B, and 100% for RSV. The prevalence and bias-adjusted kappa was 0.98 for influenza A, 0.99 for influenza B, and 0.97 for RSV. In a sensitivity analysis, the positive percent agreement values were significantly higher during the non-influenza season than the influenza season for influenza B and RSV.CONCLUSIONS:The Xpert ? Flu/RSV and Allplex ? RP1 demonstrated a high diagnostic agreement for all three viruses assessed. The seasonal variation in the positive percent agreement of the two assays for influenza B and RSV may have been due to lower numbers assessed, variability in the virology of infections outside the peak season, or changes in the physiology of the infected host in different seasons.? 2020 The Authors. Influenza and Other Respiratory Viruses published by John Wiley & Sons Ltd.
机译：背景技术基于逆转录 - 聚合酶链反应（RT-PCR）的分子测定为检测呼吸道病原体的可靠结果提供了可靠的结果，尽管诊断协议变化。本研究确定了RT-PCR测定（XPERT？FURS / RSV VS ALLEXLEXLEXEXRAMP1）在临床实践中检测流感A，流感B和呼吸道合胞病毒（RSV）之间的协议。方法：我们回顾性地确定了914名患者遭遇用xpert测试？流感/ RSV和ALLPLEX？ RP1于2015年10月和2019年9月在澳大利亚新南威尔士州的七家医院进行。使用阳性百分比协议，负百分比协议和普遍存在和偏见调整的Kappa.Results评估两种测定的诊断协议：较阳性百分比为流感A，甲型流感B的87.5％，RSV为77.8％ 。对于流感A，99.9％的甲型流感B，99.9％，100％的RSV为负百分比的协议为99.4％。流行率和偏见调整的κ为0.98，对于流感B，0.99，0.97，RSV为0.97。在敏感性分析中，非流感季节的阳性百分比百分比值显着高于流感B和RSV的流感季节：结论：Xpert？流感/ RSV和ALLPLEX？ RP1显示了评估的所有三种病毒的高诊断协议。对于流感B和RSV的两种测定的阳性百分比的季节性变化可能是由于评估的较低数量，峰值季外感染病毒学的可变性，或者在不同季节中受感染宿主的生理学的变化。还2020作者。 John Wiley＆Sons Ltd.出版的流感和其他呼吸病毒

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