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Home collection of nasal swabs for detection of influenza in the Household Influenza Vaccine Evaluation Study

机译：家庭流感疫苗评估研究中鼻拭子的鼻拭子的汇集

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BACKGROUND:Community-based studies of influenza and other respiratory viruses (eg, SARS-CoV-2) require laboratory confirmation of infection. During the current COVID-19 pandemic, social distancing guidelines require alternative data collection in order to protect both research staff and participants. Home-collected respiratory specimens are less resource-intensive, can be collected earlier after symptom onset, and provide a low-contact means of data collection. A prospective, multi-year, community-based cohort study is an ideal setting to examine the utility of home-collected specimens for identification of influenza.METHODS:We describe the feasibility and reliability of home-collected specimens for the detection of influenza. We collected data and specimens between October 2014 and June 2017 from the Household Influenza Vaccine Evaluation (HIVE) Study. Cohort participants were asked to collect a nasal swab at home upon onset of acute respiratory illness. Research staff also collected nose and throat swab specimens in the study clinic within 7?days of onset. We estimated agreement using Cohen's kappa and calculated sensitivity and specificity of home-collected compared to staff-collected specimens.RESULTS:We tested 336 paired staff- and home-collected respiratory specimens for influenza by RT-PCR; 150 staff-collected specimens were positive for influenza A/H3N2, 23 for influenza A/H1N1, 14 for influenza B/Victoria, and 31 for influenza B/Yamagata. We found moderate agreement between collection methods for influenza A/H3N2 (0.70) and B/Yamagata (0.69) and high agreement for influenza A/H1N1 (0.87) and B/Victoria (0.86). Sensitivity ranged from 78% to 86% for all influenza types and subtypes. Specificity was high for influenza A/H1N1 and both influenza B lineages with a range from 96% to 100%, and slightly lower for A/H3N2 infections (88%).CONCLUSIONS:Collection of nasal swab specimens at home is both feasible and reliable for identification of influenza virus infections.? 2020 The Authors. Influenza and Other Respiratory Viruses Published by John Wiley & Sons Ltd.
机译：背景：基于社区的流感和其他呼吸病毒的研究（例如，SARS-COV-2）需要实验室确认感染。在目前的Covid-19大流行期间，社会疏散指南需要替代数据收集，以保护研究人员和参与者。家庭收集的呼吸样本较少资源密集型，可以在症状发作后早些时候收集，并提供低接触的数据收集手段。潜在，多年，基于社区的队列研究是一种理想的环境，以检查家庭收集的标本的效用，以便识别流感。方法：我们描述了家庭收集标本用于检测流感的可行性和可靠性。我们从2014年10月和2017年6月之间收集了数据和标本，从家庭流感疫苗评估（Hive）研究。队列参与者被要求在急性呼吸疾病发作后在家里收集鼻拭子。研究人员在7？天的一天内收集了研究诊所的鼻子和喉咙拭子标本。我们通过Cohen的Kappa估计协议，并与工作人员收集的标本相比，计算了家庭收集的敏感性和特异性。结果：通过RT-PCR测试了336个成对的员工和家庭收集的流感呼吸样本; 150名员工收集的标本对于流感A / H3N2,23的流感A / H3N2,23，用于流感B / Victoria的14个，31例为流感B / Yamagata。我们发现对流感A / H3N2（0.70）和B / yamagata（0.69）和流感A / H1N1（0.87）和B / Victoria的高协议（0.86）的高度协议。所有流感类型和亚型的敏感度范围为78％至86％。流感A / H1N1的特异性高，流感B谱系的范围为96％至100％，A / H3N2感染的96％至100％略低（88％）。结论：家中鼻拭子标本的收集既可行可靠用于鉴定流感病毒感染。 2020作者。 John Wiley＆Sons Ltd.出版的流感和其他呼吸病毒

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