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首页> 外文期刊>Microbial Cell Factories >Production of bio-xylitol from d -xylose by an engineered Pichia pastoris expressing a recombinant xylose reductase did not require any auxiliary substrate as electron donor
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Production of bio-xylitol from d -xylose by an engineered Pichia pastoris expressing a recombinant xylose reductase did not require any auxiliary substrate as electron donor

机译：由表达重组木糖还原酶的工程化毕赤酵母的D- Xylose的生物 - 木糖醇的生产不需要任何辅助基材作为电子给体

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Xylitol is a five-carbon sugar alcohol that has numerous beneficial health properties. It has almost the same sweetness as sucrose but has lower energy value compared to the sucrose. Metabolism of xylitol is insulin independent and thus it is an ideal sweetener for diabetics. It is widely used in food products, oral and personal care, and animal nutrition as well. Here we present a two-stage strategy to produce bio-xylitol from d-xylose using a recombinant Pichia pastoris expressing a heterologous xylose reductase gene. The recombinant P. pastoris cells were first generated by a low-cost, standard procedure. The cells were then used as a catalyst to make the bio-xylitol from d-xylose. Pichia pastoris expressing XYL1 from P. stipitis and gdh from B. subtilis demonstrated that the biotransformation was very efficient with as high as 80% (w/w) conversion within two hours. The whole cells could be re-used for multiple rounds of catalysis without loss of activity. Also, the cells could directly transform d-xylose in a non-detoxified hemicelluloses hydrolysate to xylitol at 70% (w/w) yield. We demonstrated here that the recombinant P. pastoris expressing xylose reductase could transform d-xylose, either in pure form or in crude hemicelluloses hydrolysate, to bio-xylitol very efficiently. This biocatalytic reaction happened without the external addition of any NAD(P)H, NAD(P) , and auxiliary substrate as an electron donor. Our experimental design & findings reported here are not limited to the conversion of d-xylose to xylitol only but can be used with other many oxidoreductase reactions also, such as ketone reductases/alcohol dehydrogenases and amino acid dehydrogenases, which are widely used for the synthesis of high-value chemicals and pharmaceutical intermediates.
机译：木糖醇是一种五碳糖醇，具有许多有益的健康特性。它与蔗糖几乎相同的甜味，但与蔗糖相比具有较低的能量值。木糖醇的代谢是胰岛素独立的，因此它是糖尿病患者的理想甜味剂。它广泛用于食品，口服和个人护理，以及动物营养。在这里，我们使用表达异源木糖还原酶基因的重组Pichia牧场来提出一种两级策略来生产来自D-木糖的生物木糖醇。首先通过低成本，标准程序产生重组P.牧场菌细胞。然后将细胞用作催化剂以使来自D-木糖的生物 - 木糖醇。从P.毛皮炎和B.枯草芽孢杆菌表达Xyl1的Pichia Pastoris证明了生物转化在两小时内高达80％（w / w）转化非常有效。整个细胞可以重新使用多轮催化而不会丧失活动。此外，细胞可以直接将非解毒的半纤维素中的D-木糖转化为70％（w / w）产率的含氟盐。我们在此证明表达木糖还原酶的重组P.牧场可以以纯的形式或粗糙的半纤维素水解，以非常有效地将D-木糖转化为生物 - 木糖醇。这种生物催化反应发生而没有外部添加任何NAD（P）H，NAD（P）和辅助基板作为电子给体。这里报道的我们的实验设计和结果不限于D-木糖对木糖醇的转化，但也可以与其他许多氧化还原酶反应一起使用，例如酮还原酶/醇脱氢酶和氨基酸脱氢酶，其广泛用于合成高价值化学品和药物中间体。

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