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首页> 外文期刊>Plant Protection Science >Genotypic differentiation of Monilinia spp. populations in Serbia using a high-resolution melting (HRM) analysis
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Genotypic differentiation of Monilinia spp. populations in Serbia using a high-resolution melting (HRM) analysis

机译：Monilinia SPP的基因型分化。 塞尔维亚使用高分辨率熔化（HRM）分析的群体

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Monilinia laxa, Monilinia fructicola and Monilinia fructigena are the?three main causal agents of?brown rot, which is one of? the most important diseases of? stone fruits in? pre- and postharvest conditions. Nowadays, the? need for?the precise genotyping of?these Monilinia species in?terms of?the genetic diversity of?their populations or differences in? their pathogenicity and host range is a prerequisite for? any efficient disease management. In? our study, the? genetic structure of?Monilinia populations in?Serbia from three geographically distinct regions was?investigated employing a high-resolution melting (HRM) analysis which is a sensitive and rapid molecular approach in fungal genotyping and diagnostics. Using species-specific primer pairs genotype-specific HRM melting curve profiles were generated allowing to?efficiently decipher the?genetic diversity of?the Monilinia populations. The?Monilinia genotypes could be easily distinguished according to?their melting curves. The?isolates from the?northern region were assigned to distinct genotypes and grouped rather independently compared to?the isolates of?the other two regions among all three tested Monilinia spp. M. fructicola and M. fructigena showed a higher genetic diversity among their populations (44%) compared with the?genetic diversity among the?M. laxa populations (7%). In?contrast, the?genetic variance within the?pathogen populations was?higher in?the case of?M. laxa (93%). Our data revealed an?absence of?host specificity in?the Monilinia spp. populations.
机译：monilinia laxa，monilinia fructicola和monilinia fructiena是什么？棕色腐烂的三个主要因果剂，这是一个？最重要的疾病？石头水果？前后条件。如今，？需要吗？这种monilinia物种的精确基因分型？术语？遗传多样性？他们的群体或差异？他们的致病性和宿主范围是一个先决条件？任何有效的疾病管理。在？我们的研究，？来自三个地理上不同地区的塞尔维亚的遗传结构？来自三个地理上不同地区的塞尔维亚群体是αscapeated采用高分辨率熔化（HRM）分析，这是真菌基因分型和诊断的敏感和快速的分子方法。使用特异性引物对基因型特异性HRM熔化曲线曲线允许？有效地破译？遗传多样性？Monilinia群体。 Monilinia基因型可以很容易地根据其熔化的曲线区分。北部地区的分离株分配给不同的基因型，并与β相比分组相当独立于α？另外三个测试的monilinia spp中的其他两个区域。 M. Fructicola和M. Fructigena在他们的群体中展现了更高的遗传多样性（44％），而遗传多样性在？M之间的遗传多样性。拉萨萨人口（7％）。在？对比中，α病原体群体内的遗传方差？较高？壳的情况。 Laxa（93％）。我们的数据显示出缺乏？宿主特异性？Monilinia SPP。人口。

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