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首页> 外文期刊>BMC Genomics >LCM and RNA-seq analyses revealed roles of cell cycle and translational regulation and homoeolog expression bias in cotton fiber cell initiation
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LCM and RNA-seq analyses revealed roles of cell cycle and translational regulation and homoeolog expression bias in cotton fiber cell initiation

机译：LCM和RNA-SEQ分析显示细胞周期和翻译规则和同源性表达偏差在棉纤维细胞启动中的作用

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Cotton fibers provide a powerful model for studying cell differentiation and elongation. Each cotton fiber is a singular and elongated cell derived from epidermal-layer cells of a cotton seed. Efforts to understand this dramatic developmental shift have been impeded by the difficulty of separation between fiber and epidermal cells. Here we employed laser-capture microdissection (LCM) to separate these cell types. RNA-seq analysis revealed transitional differences between fiber and epidermal-layer cells at 0 or 2?days post anthesis. Specifically, down-regulation of putative cell cycle genes was coupled with upregulation of ribosome biosynthesis and translation-related genes, which may suggest their respective roles in fiber cell initiation. Indeed, the amount of fibers in cultured ovules was increased by cell cycle progression inhibitor, Roscovitine, and decreased by ribosome biosynthesis inhibitor, Rbin-1. Moreover, subfunctionalization of homoeologs was pervasive in fiber and epidermal cells, with expression bias towards 10% more D than A homoeologs of cell cycle related genes and 40–50% more D than A homoeologs of ribosomal protein subunit genes. Key cell cycle regulators were predicted to be epialleles in allotetraploid cotton. MYB-transcription factor genes displayed expression divergence between fibers and ovules. Notably, many phytohormone-related genes were upregulated in ovules and down-regulated in fibers, suggesting spatial-temporal effects on fiber cell development. Fiber cell initiation is accompanied by cell cycle arrest coupled with active ribosome biosynthesis, spatial-temporal regulation of phytohormones and MYB transcription factors, and homoeolog expression bias of cell cycle and ribosome biosynthesis genes. These valuable genomic resources and molecular insights will help develop breeding and biotechnological tools to improve cotton fiber production.
机译：棉纤维为研究细胞分化和伸长率提供了强大的模型。每个棉纤维是衍生自棉籽的表皮层细胞的奇异和细长的细胞。通过纤维和表皮细胞分离的难度来阻碍理解这种急剧发育转变的努力。在这里，我们采用激光捕获的微小切割（LCM）来分离这些细胞类型。 RNA-SEQ分析显示出在开花后0或2天的纤维和表皮层细胞之间的过渡差异。具体地，调节细胞周期基因的下调与核糖体生物合成和翻译相关基因的上调，这可能表明它们在纤维细胞引发中的各自作用。实际上，通过细胞周期进展抑制剂，roscovitine，通过核糖体生物合成抑制剂，RBIN-1减少培养胚珠中的纤维量。此外，同种晶状体的副官能化在纤维和表皮细胞中是普遍存在的，表达偏差比细胞周期相关基因的同源表现出的10％，而不是核糖体蛋白亚基基因的同源物学。预计键细胞周期调节剂是同种异体四倍体棉中的表现。 MyB-转录因子基因显示纤维和胚珠之间的表达分歧。值得注意的是，许多植物激素相关基因在胚珠中升高并在纤维中下调，表明对纤维细胞发育的空间效应。纤维细胞启动伴随着细胞周期停滞，耦合有活性性核糖体生物合成，植物激素和MYB转录因子的空间调节，以及细胞周期和核糖体生物合成基因的同种表达偏差。这些有价值的基因组资源和分子见解将有助于开发育种和生物技术工具，以改善棉纤维生产。

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