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Detection of biofilm formation among Pseudomonas aeruginosa isolated from burn patients

机译：烧伤患者铜绿假单胞菌中生物膜形成的检测

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Background Pseudomonas aeruginosa is an opportunistic bacterium that rapidly colonised on the exacerbated skin surface after the burn injury. Along with inherent resistance to many antibiotics, it is also a good biofilm producer that aggravates the condition of burns patients by slow or non-responsive to antibiotics. Therefore, immediate attention must be taken otherwise the condition of patients may become life-threatening. Hence, this cross-sectional study was conducted to know the current scenario of antimicrobial profile and biofilm properties of Pseudomonas aeruginosa isolated from burn patients in Nepal Cleft and Burn Centre, Kirtipur Hospital. Methods Wound swab or pus samples were taken from 344 patients through the Levine technique or syringe, respectively. The standard laboratory protocol was followed for the identification of Pseudomonas aeruginosa . The antibiotic susceptibility test of isolates was performed by using the Kirby-Bauer disc diffusion method as per the CLSI 2018 guidelines. Biofilm production was accessed through the tube method and microtiter plate assay. Results All together 60 Pseudomonas aeruginosa were isolated from a total of 275 isolates. The antibiotic susceptibility profile showed that more than 50% of isolates were non-susceptible among the tested antibiotics and shifting of antibiotic choice to piperacillin-tazobactam from traditional amikacin, ciprofloxacin and imipenem. The weak agreement of Kappa statistics indicates that biofilm procurement from the tube method and microtiter plate assay was insignificant. Approximately 25% of P. aeruginosa isolates were biofilm producers and there was no relation between biofilm production and antibiotic susceptibility patterns (χ2?=?0.587). Conclusion Pseudomonas aeruginosa is a common isolate from burn wound infections. There is a shifting in antibiotic choice and more than half of the isolates were antibiotic resistant; however, no relation between biofilm formation and multidrug resistant profile of isolates was found.
机译：背景技术假单胞菌是一种机会主义的细菌，在燃烧损伤后迅速殖民迅速殖民。随着对许多抗生素的固有抗性，它也是一种很好的生物膜生产商，通过慢性或非响应抗生素来加剧烧伤患者的状况。因此，否则必须立即注意患者的状况可能会成为危及生命的危险性。因此，进行这种横截面研究以了解当前从尼泊尔裂缝和烧伤中心烧伤患者中分离的假药型铜绿假单胞菌的抗微生物剖面和生物膜特性的场景。方法伤口拭子或PUS样品分别通过Levine技术或注射器从344名患者中取出。遵循标准实验室方案，用于鉴定假单胞菌铜绿假单胞菌。通过使用CLSI 2018指南使用Kirby-Bauer光盘扩散方法进行分离株的抗生素敏感性试验。通过管方法和微量滴定板测定进入生物膜生产。结果均在一起60孔核苷酸分离出总共275分离物。抗生素敏感性曲线显示，超过50％的分离物在测试的抗生素和抗生素选择中的抗生素选择从传统的Amikacin，Ciprofloxacin和Imipenem的转化。 Kappa统计的弱吻合表明，来自管方法和微量滴定板测定的生物膜采购微不足道。大约25％的铜绿假单胞菌分离株是生物膜生产商，生物膜生产和抗生素敏感性模式之间没有关系（χ2？= 0.587）。结论假单胞菌铜绿假单胞菌是一种来自伯爵伤口感染的常见分离物。抗生素选择中存在差异，超过一半的分离物是抗生素的抗性;然而，发现生物膜形成与分离株的多药抗性曲线之间无关。

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