...
  • 主题
高级搜索  >
历史搜索 清空历史
首页> 外文期刊>Analytical Sciences >Study on the Biosensor Based on Biomimetic PDA Vesicles Fluorescence Resonance Energy Transfer for the Determination of Ovarian Cancer Marker miRNA-21
【24h】

Study on the Biosensor Based on Biomimetic PDA Vesicles Fluorescence Resonance Energy Transfer for the Determination of Ovarian Cancer Marker miRNA-21

机译：基于仿生PDA囊泡荧光共振能量转移的生物传感器研究卵巢癌标志MiRNA-21的生物传感器

获取原文
   

获取外文期刊封面封底 >>

       
页面导航
  • 摘要
  • 著录项
  • 相似文献
  • 相关主题

摘要

In recent years, more and more research is being conducted on microRNAs and their involvement in the regulation of autophagy phagocytosis which is closely related to tumor growth. MicroRNA-21 is a kind of small RNA that can regulate gene expression and plays a significant role in autophagy of tumor cells. But the detection of microRNAs had always been a problem in the field of biological analysis. In this study, we designed a new fluorescent sensor for the detection of miRNA-21. The sensor was based on the successful signal reporting by E36-encapsulated vesicles and the specific interaction between E36 and miRNA-21. In the presence of miRNA, the E36/miRNA-21 complex formed and served as a donor molecule inside the acceptor PDA vesicles to amplify the fluorescence through FRET. Additionally, the sensor was applied to detect miRNA-21 in complex biological samples with satisfactory results.
机译：近年来，越来越多的研究正在小罗车上进行，他们参与了与肿瘤生长密切相关的自噬吞噬作用的调节。 MicroRNA-21是一种可以调节基因表达的小RNA，并在肿瘤细胞的自噬中起着重要作用。 但是微小RNA的检测始终是生物分析领域的问题。 在这项研究中，我们设计了一种用于检测miRNA-21的新型荧光传感器。 传感器基于E36封装的囊泡的成功信号报告和E36和miRNA-21之间的特异性相互作用。 在miRNA的存在下，形成的E36 / miRNA-21复合物在受体PDA囊泡内部形成并用作供体分子，以通过FRET扩增荧光。 另外，施用传感器以检测复杂生物样品中的miRNA-21，结果令人满意。

著录项

相似文献

  • 外文文献
  • 中文文献
  • 专利
获取原文

客服邮箱：kefu@zhangqiaokeyan.com

京公网安备：11010802029741号 ICP备案号：京ICP备15016152号-6 六维联合信息科技 (北京) 有限公司©版权所有

  • 客服微信

  • 服务号