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首页> 外文期刊>International Journal of Pharmacology >Antibiofilm Activity of Oxytetracycline Loaded Calcium Carbonate Aragonite Loaded Nanoparticle Against Corynebacterium pseudotuberculosis
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Antibiofilm Activity of Oxytetracycline Loaded Calcium Carbonate Aragonite Loaded Nanoparticle Against Corynebacterium pseudotuberculosis

机译：催产素的抗生素碳酸钙碳酸钙碳酸钙碳酸钙的活性纳米粒子对抗棒状菌染色菌。

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Background and Objective: Corynebacterium pseudotuberculosis is the causative agent of caseous lymphadenitis, a zoonotic bacterial disease of small ruminants. The disease is unresponsive to antibiotic treatment due to the intracellular nature of the bacteria, thick purulent exudates, antibiotic resistance and formation of biofilms. The objective of this study was to evaluate the antibiofilm activity of oxytetracycline in free (OTC) and cockle shell loaded forms (OTC-CS-CaCO 3 NP) against C. pseudotuberculosis . Materials and Methods: The Minimum Inhibitory Concentration (MIC) and Minimum Biofilm Eradication Concentration (MBEC) of oxytetracycline in free and cockle shell loaded forms against C. pseudotuberculosis were determined by the microtiter plate assay method. Biofilm formation and inhibition assays were studied spectrophotometrically using the crystal violet assay method. TEM, SEM and fluorescent microscopy were used to assess the antibacterial and antibiofilm effect against C. pseudotuberculosis . Results: The MIC of OTC and OTC-CS-CaCO 3 NP against C. pseudotuberculosis were 125 and 500 μg L ? 1 , respectively while the MBEC was 250 and 2000 μg L ? 1 . TEM analysis showed OTC-CS-CaCO 3 NP disrupted cell membrane and formation of vacuoles in C. pseudotuberculosis . Conclusion: Microscopic analysis of the biofilms revealed OTC-CS-CaCO 3 NP caused significant morphological changes compared to OTC. These results suggest the potential applicability of OTC-CS-CaCO 3 NP for the treatment of caseous lymphadenitis.
机译：背景和目的：棒状杆菌是对脑梗塞淋巴结炎的致病剂，是小反刍动物的一根细菌疾病。由于细菌的细胞内性质，厚化脓性渗出物，抗生素抗性和生物膜形成，该疾病对抗生素治疗无响应。本研究的目的是评估无氧杂环素的抗生素活性（OTC）和Cockle Shell负载的形式（OTC-CS-CaCO 3 NP）对C.Pseudubliss。材料和方法：通过微量滴定板测定法测定单次抑制与C.Pseudubulculosis的最小抑制浓度（MIC）和催眠素的最小生物膜消除浓度（MBEC）。使用晶体紫测定法在分光光度法研究生物膜形成和抑制测定。用于评估针对抗菌和抗菌和抗菌菌的抗菌和抗菌菌对C.假偶霉菌的影响。结果：OTC和OTC-CS-CACO 3 NP的MIC针对C.Pseudotuberculosis为125和500μgL？在MBEC为250和>2000μgl时，分别为1，分别为1。 1。 TEM分析显示OTC-CS-CaCO 3 NP中断细胞膜和C.Psudotublosis中的液压液形成。结论：生物膜的微观分析显示出OTC-CS-CACO 3 NP与OTC相比引起了显着的形态变化。这些结果表明OTC-CS-CACO 3 NP对脑舌淋巴结炎治疗的潜在适用性。

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