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首页> 外文期刊>Frontiers in Cell and Developmental Biology >3D Cartilage Regeneration With Certain Shape and Mechanical Strength Based on Engineered Cartilage Gel and Decalcified Bone Matrix
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3D Cartilage Regeneration With Certain Shape and Mechanical Strength Based on Engineered Cartilage Gel and Decalcified Bone Matrix

机译：3D基于工程软骨凝胶和脱钙骨基质的某些形状和机械强度的软骨再生

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摘要

Scaffold-free cartilage-sheet technology can stably regenerate high-quality cartilage tissue in vivo. However, uncontrolled shape maintenance and mechanical strength greatly hinder its clinical translation. Decalcified bone matrix (DBM) has high porosity, a suitable pore structure, and good biocompatibility, as well as controlled shape and mechanical strength. In this study, cartilage sheet was prepared into engineered cartilage gel (ECG) and combined with DBM to explore the feasibility of regenerating 3D cartilage with controlled shape and mechanical strength. The results indicated that ECG cultured in vitro for 3 days (3d) and 15 days (15d) showed good biocompatibility with DBM, and the ECG–DBM constructs successfully regenerated viable 3D cartilage with typical mature cartilage features both in nude mice and autologous goats. Additionally, the regenerated cartilage had comparable mechanical properties to native cartilage and maintained its original shape. To further determine the optimal seeding parameters for ECG, the 3d ECG regenerated using human chondrocytes was diluted in different concentrations (1:3, 1:2, 1:1) for seeding and in vivo implantation. The results showed that the regenerated cartilage in the 1:2 group exhibited better shape maintenance and homogeneity than the other groups. The current study established a novel mode of 3D cartilage regeneration based on the design concept of steel (DBM)-reinforced concrete (ECG) and successfully regenerated homogenous and mature 3D cartilage with controlled shape and mechanical strength, which hopefully provides an ideal cartilage graft for the repair of various cartilage defects.
机译：无脚手架软骨片技术可以在体内稳定再生高质量的软骨组织。但是，不受控制的形状维护和机械强度大大阻碍了其临床翻译。癸化骨基质（DBM）具有高孔隙率，合适的孔结构，以及良好的生物相容性，以及控制的形状和机械强度。在该研究中，软骨片被制备到工程化软骨凝胶（ECG）中并与DBM结合，探讨具有受控形状和机械强度的再生3D软骨的可行性。结果表明，在体外培养3天（3D）和15天（15D）的ECG显示出与DBM的良好生物相容性，ECG-DBM构建成功再生的可行性3D软骨，裸鼠和自体山羊典型的成熟软骨特征。另外，再生软骨与天然软骨具有相当的机械性能，并保持其原始形状。为了进一步确定ECG的最佳播种参数，使用人软骨细胞再生的3D ECG以不同浓度（1：3,1：2,1：1）稀释以进行播种和体内植入。结果表明，1：2组中的再生软骨表现出比其他组更好的形状维护和均匀性。目前的研究建立了一种基于钢（DBM）设计概念的三维软骨再生模式 - 重新凝固混凝土（ECG），并成功再生均匀和成熟的3D软骨，具有受控形状和机械强度，希望提供理想的软骨移植物修复各种软骨缺陷。

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