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首页> 外文期刊>JMIR public health and surveillance. >YouTube Videos Demonstrating the Nasopharyngeal Swab Technique for SARS-CoV-2 Specimen Collection: Content Analysis
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YouTube Videos Demonstrating the Nasopharyngeal Swab Technique for SARS-CoV-2 Specimen Collection: Content Analysis

机译：YouTube视频演示了SARS-COV-2标本收集的鼻咽拭子技术：内容分析

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Background Real-time polymerase chain reaction using nasopharyngeal swabs is currently the most widely used diagnostic test for SARS-CoV-2 detection. However, false negatives and the sensitivity of this mode of testing have posed challenges in the accurate estimation of the prevalence of SARS-CoV-2 infection rates. Objective The purpose of this study was to evaluate whether technical and, therefore, correctable errors were being made with regard to nasopharyngeal swab procedures. Methods We searched a web-based video database (YouTube) for videos demonstrating SARS-CoV-2 nasopharyngeal swab tests, posted from January 1 to May 15, 2020. Videos were rated by 3 blinded rhinologists for accuracy of swab angle and depth. The overall score for swab angle and swab depth for each nasopharyngeal swab demonstration video was determined based on the majority score with agreement between at least 2 of the 3 reviewers. We then comparatively evaluated video data collected from YouTube videos demonstrating the correct nasopharyngeal swab technique with data from videos demonstrating an incorrect nasopharyngeal swab technique. Multiple linear regression analysis with statistical significance set at P=.05 was performed to determine video data variables associated with the correct nasopharyngeal swab technique. Results In all, 126 videos met the study inclusion and exclusion criteria. Of these, 52.3% (66/126) of all videos demonstrated the correct swab angle, and 46% (58/126) of the videos demonstrated an appropriate swab depth. Moreover, 45.2% (57/126) of the videos demonstrated both correct nasopharyngeal swab angle and appropriate depth, whereas 46.8% (59/126) of the videos demonstrated both incorrect nasopharyngeal swab angle and inappropriate depth. Videos with correct nasopharyngeal swab technique were associated with the swab operators identifying themselves as a medical professional or as an Ear, Nose, Throat–related medical professional. We also found an association between correct nasopharyngeal swab techniques and recency of video publication date (relative to May 15, 2020). Conclusions Our findings show that over half of the videos documenting the nasopharyngeal swab test showed an incorrect technique, which could elevate false-negative test rates. Therefore, greater attention needs to be provided toward educating frontline health care workers who routinely perform nasopharyngeal swab procedures.
机译：背景技术使用鼻咽拭子的实时聚合酶链反应是目前最广泛使用的SARS-COV-2检测诊断测试。然而，错误的否定和这种测试模式的敏感性在准确估算SARS-COV-2感染率的准确估算中引起了挑战。目的是本研究的目的是评估是否正在关于鼻咽拭子程序进行矫正错误。方法我们搜索了一个基于Web的视频数据库（YouTube），用于演示SARS-COV-2鼻咽拭子测试，从1月1日至5月15日起，在3月1日至5月15日起。视频被3个盲文学学家评分为3个盲目的哨兵，用于拭子角度和深度的准确性。基于3个评论者中至少2个之间的一致性，确定了每个鼻咽拭子演示视频的拭子角度和拭子深度的总分。然后，我们比较评估从YouTube视频收集的视频数据，演示正确的鼻咽拭子技术，从视频中的数据显示出不正确的鼻咽拭子技术。进行了在P = .05处设定的统计显着性的多元线性回归分析，以确定与正确的鼻咽拭子技术相关的视频数据变量。结果所有，126个视频符合研究纳入和排除标准。其中，所有视频的52.3％（66/126）展示了正确的拭子角度，46％（58/126）的视频演示了适当的拭子深度。此外，视频的45.2％（57/126）证明了正确的鼻咽拭子角度和适当的深度，而46.8％（59/126）的视频显示出不正确的鼻咽拭子角度和不恰当的深度。具有正确的鼻咽拭子技术的视频与拭子运营商识别自己作为医疗专业人员或耳朵，鼻子，喉咙相关医学专业人员有关。我们还发现了矫正鼻咽拭子技术与视频出版日期的新近记录之间的关联（相对于2020年5月15日）。结论我们的调查结果表明，记录了鼻咽拭子测试的一半视频显示出不正确的技术，这可能提升假阴性测试率。因此，需要更加关注教育前线保健工作者，这些人经常进行鼻咽拭子程序。

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