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首页> 外文期刊>Journal of Behavioral and Brain Science >Experimental Study on the Effect of Acupuncture on ERM/PI3/Akt Signal Pathway in Rats with Ischemic Stroke
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Experimental Study on the Effect of Acupuncture on ERM/PI3/Akt Signal Pathway in Rats with Ischemic Stroke

机译：针刺对缺血性卒中大鼠ERM / PI3 / AKT信号途径影响的实验研究

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Objective: To observe the effect of acupuncture on the expression of ERM/PI3K/Akt signaling pathway in the brain tissue around the infarct focus of ischemic stroke model rats. Methods: According to the block randomization method, 80 male SD rats were divided into normal group (n = 10), sham operation group (n = 24), model group (n = 23) and acupuncture group (n = 23). The normal group was bred routinely without any intervention. In the sham operation group, only the skin was cut to find and the nerves and blood vessels were stripped and sutured. The model group and the acupuncture group were sutured to prepare the MCAO model. After the model was successfully established, acupuncture of the acupuncture group rats was given once a day, and the needles were kept for 30 minutes each time. The rats in the sham operation group and the model group only imitated capture and return, and were treated with acupuncture for 2 consecutive courses, each course was 5 days, and the two courses were rested for 2 days. After that, Western blot was used to detect ERM, changes in the concentration of PI3K and Akt proteins. Results: Compared with the normal group, the expression levels of ERM, PI3K, and Akt proteins in the sham operation group were reduced. Compared with the sham operation group, the expression levels of ERM, PI3K, and Akt proteins in the model group were significantly down-regulated. Compared with the model group, the protein expression levels of ERM, PI3K and Akt were significantly up-regulated in the acupuncture group, and the difference was statistically significant (P < 0.05). Conclusion: Acupuncture can significantly up-regulate the expression of ERM, PI3K and Akt proteins, and its effect mechanism may be related to the transduction of ERM/PI3K/Akt signaling pathway in cells.
机译：目的：观察针灸对缺血性脑卒中模型大鼠梗死焦点脑组织ERM / PI3K / AKT信号通路表达的影响。方法：根据嵌段随机化方法，将80只雄性SD大鼠分为正常组（n = 10），假手术组（n = 24），模型组（n = 23）和针灸组（n = 23）。正常组常规育种而没有任何干预。在假手术组中，只切除皮肤以发现，剥离并缝合神经和血管。模型组和针灸组被缝合以准备MCAO模型。成功建立了模型后，针灸群大鼠每天给药一次，每次都保持皮革30分钟。假手术组和模型组中的大鼠仅仿捕获和返回，并用针灸治疗连续2个课程，每门课程为5天，两种课程休息2天。之后，使用Western印迹来检测ERM，PI3K和AKT蛋白的浓度变化。结果：与正常组相比，假手术组中ERM，PI3K和AKT蛋白的表达水平降低。与假手术组相比，模型组中ERM，PI3K和AKT蛋白的表达水平显着下调。与模型组相比，在针灸组中，ERM，PI3K和AKT的蛋白质表达水平显着上调，差异有统计学意义（P <0.05）。结论：针灸可显着上调ERM，PI3K和AKT蛋白的表达，其效应机制可能与细胞中ERM / PI3K / AKT信号通路的转导进行相关。

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