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首页> 外文期刊>Journal of Taibah University for Science >Industrially relevant pectinase production from Aspergillus parvisclerotigenus KX928754 using apple pomace as the promising substrate
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Industrially relevant pectinase production from Aspergillus parvisclerotigenus KX928754 using apple pomace as the promising substrate

机译：工业相关的果胶酶生产从Aspergillus parvisclerotigenus KX928754使用Apple Pomace作为有前途的基材

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摘要

The present study was focused on the potentiality of agro-based residues for the production of pectinase to meet the growing market demand by improving the yield with low cost of production. Among the agro-based residues used for the production of pectinase, apple pomace was able to produce the maximum of 1366.30 ± 36.71 U/ml using Aspergillus parvisclerotigenus KX928754 in liquid static surface fermentation, followed by sugarcane bagasse (973.12 ± 22.43 U/ml) and used tea (686.7 ± 45.06 U/ml). The process parameters optimization using a single variable at a time affirmed that pH 7.0, incubation period of 168 h, 30°C temperature, sucrose 2% as carbon source and peptone 3% as nitrogen source was found to be optimum for better production. The crude filtrate was purified by precipitation, dialyzed, eluted on Sephadex G-100 column followed by lyophilization and stored at ?20°C. A. parvisclerotigenus KX928754 pectinase was purified to 2.10-fold, 2.91% of yield rate and having a specific activity of 1081.66 U/mg. Moreover, the electrophoretic analysis through sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS-PAGE) revealed 37.4 kDa of protein from the purified pectinase. Thus, the use of apple pomace as a substrate for scaling up pectinase with efficient recovery could reduce the price of the enzyme and increase its avenue for different industrial exploitation.
机译：本研究重点是通过提高生产成本低的产量来培养基于农业基础酶的潜在残留物，以满足日益增长的市场需求。在用于生产果胶酶的基于农业的残留量中，苹果渣能够在液态静电表面发酵中使用曲霉菌菌，然后在液体静电表面发酵中产生最大1366.30±36.71u / ml，其次是甘蔗面包（973.12±22.43 U / ml）并用过茶（686.7±45.06 u / ml）。一次使用单个变量的过程参数优化肯定是pH 7.0，孵育时间为168小时，30℃，蔗糖2％作为碳源和蛋白胨3％作为氮源，以最佳的生产。通过沉淀，透析析出粗滤液，在Sephadex G-100柱上洗脱，然后冻干并储存在20℃。 A. Parvisclerotigenus KX928754果胶酶纯化至2.10倍，2.91％的屈服率，具有1081.66u / mg的特异性活性。此外，通过十二烷基硫酸钠聚丙烯酰胺凝胶电泳（SDS-PAGE）的电泳分析揭示了来自纯化果胶酶的37.4kDa蛋白质。因此，使用苹果渣作为用于缩放果胶酶的基质，具有有效的恢复可以降低酶的价格，并增加其不同工业剥削的大道。

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