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首页> 外文期刊>Journal of Clinical and Diagnostic Research >Biochemical Estimation in an Acute Toxicity Study of Narayana Chenduram- A Siddha Formulation
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Biochemical Estimation in an Acute Toxicity Study of Narayana Chenduram- A Siddha Formulation

机译：Narayana Chenduram-一种急性毒性研究的生物化学估算 - 一种Siddha制剂

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Chendurams are more potent and its potency is further increased when combined with herbal juices. Chenduram is known to be effective when it is given at low concentration. Narayana Chenduram (NC) is a metal based siddha formulation that contains heavy metals like mercury, cinnabar, arsenic along with sulphur/sulphides. NC is used to treat Parkinson’s disease along with the polyherbal formulation Athimathura Gritham (AG). The present study was undertaken to evaluate the acute oral toxicity of NC.Aim: To evaluate the health status of the animals under acute oral toxicity study of NC. Biochemical estimation of heavy metals in liver, kidney, brain and serum was carried out. Histopathological study was also performed in liver and kidney.Materials and Methods: The present experimental study was conducted in Department of Anatomy, University of Madras, Chennai, India from October to December 2009. The heavy metals present in NC was analysed by Inductively Coupled Plasma Optical Emission Spectrophotometry (ICP-OES) using acid digestion method. A single oral dose acute toxicity study of NC was conducted using acute toxic class method as per OECD (Organisation for Economic Cooperation and Development) Guidelines for the Testing of Chemicals. It was done using limit test method. The study was conducted by giving NC at a single dose of 2000 mg/kg body weight mixed in 10 mL of honey/kg body weight and rats were observed for 14 days for toxic signs. Total five animals were tested under this method to determine Lethal Dose 50 (LD50). On day 15, the distribution of heavy metals in liver, kidney, brain and serum was determined by ICP-OES using acid digestion method and was compared with control. Food and water intake, body weight were recorded before and after drug administration as per the guidelines. Histopathological examination of liver and kidney was performed in the same animals and compared with control. To find the effect of given adjuvant (honey) under acute oral toxicity study, NC at a single dose of 1000 mg mixed with 10 mL of honey/kg body weight was administered to one group of animals and NC at a single dose of 1000 mg mixed with 10 mL of sesame oil/kg body weight was given orally to another group of animals. After administration of test drug, the rats were observed for 14 days for toxic signs and on 15th day they were sacrificed to study histological changes in kidney and liver among these two groups. Adjuvant treated control group of animals were administered only with 10 mL of honey at a single dose and observed for 14 days and sacrificed on day 15 to study histology of liver and kidney and compared with control.Results: No mortality was observed at a single dose of 2000 mg/kg under acute toxicity study. Hence, LD50 was greater than 2000 mg/kg body weight. ICP-OES analysis showed that the mean concentration of mercury was five times more than that of mean concentration of arsenic in the given sample weight of NC. Under acute toxicity study, after oral administration of NC at a single dose of 2000 mg/kg body weight, serum showed more significant accumulation of mercury than arsenic when compared to control groups. A single dose of 2000 mg NC produced hepato-toxicity and renal toxicity. At a single dose of NC at 1000 mg mixed with 10 mL honey showed less histopathological changes when compared with NC mixed with sesame oil. Adjuvant (honey) treated group did not show any histopathological toxicity in liver and kidney when compared with control.Conclusion: Though there was no mortality at a single dose of 2000 mg/kg body weight, serum showed marked accumulation of mercury that indicates toxicity. It produced signs of histopathological toxicity in liver and kidney. Dose dependent change was observed. It is recommended to use Chenduram only at low dose which is effective. It was proved in our previous work that NC at therapeutic dose level along with AG was effective in rotenone induced Parkinsonism in Wistar albino rats.
机译：Chendurams更有效，与草药果汁结合时，其效力进一步增加。已知在低浓度下给出时是有效的Chenduram。 Narayana Chenduram（NC）是一种基于金属的SiddHA配方，含有汞，甘露糖，砷和硫化物等重金属。 NC用于治疗Parkinson的疾病以及聚鼠制剂Athimathura Gritham（AG）。本研究进行了评价NC.aim的急性口腔毒性：评估急性口腔毒性研究下的动物的健康状况。进行了肝，肾，脑和血清重金属的生化估计。组织病理学研究也在肝脏和肾脏中进行.8099年10月至12月，在印度钦奈大学的Madras大学，NC中的重金属，通过电感耦合等离子体分析了本实验研究，在印度Madras大学的解剖学系进行了实验研究。使用酸消化方法的光发射分光光度法（ICP-OES）。根据经合组织（经济合作与发展组织）使用急性毒性级别方法进行NC的单一口服剂量急性毒性研究，用于测试化学品的准则。它是使用限制测试方法完成的。该研究通过在10ml蜂蜜/ kg体重混合的2000mg / kg体重中的单一剂量赋予NC进行NC进行，并且观察大鼠14天的有毒迹象。在该方法下测试总共五种动物以确定致死剂量50（LD50）。在第15天，使用酸消化方法通过ICP-OES测定肝脏，肾，脑和血清中重金属的分布，并与对照进行比较。食物和水摄入量，在指导方针之前和药物管理前后记录体重。在相同的动物中进行肝脏和肾的组织病理学检查并与对照进行比较。为了发现给定佐剂（蜂蜜）在急性口腔毒性研究下的效果，用10mL蜂蜜/ kg体重混合的单剂量为1000mg的NC以1000mg的单剂量给予一组动物和NC与10ml芝麻油/ kg体重混合给另一组动物。在给予试验药物后，将大鼠观察到有毒迹象14天，并在第15天中牺牲它们在这两组中研究肾脏和肝脏的组织学变化。佐剂处理的对照组动物仅用10ml蜂蜜以单一剂量施用，并观察到14天，并在第15天处死以研究肝肾的组织学，并与对照进行比较。结果：在单个剂量中没有观察到死亡率急性毒性研究中2000毫克/千克。因此，LD50大于2000mg / kg体重。 ICP-OES分析表明，汞的平均浓度比NC的给定样品重量中的砷的平均浓度大于5倍。在急性毒性研究下，在单一剂量的2000mg / kg体重时口服NC后，与对照组相比，血清表现出比砷的更大积累而大于砷。单剂量为2000毫克NC产生肝毒性和肾毒性。在与芝麻油混合的NC混合时，在1000mg的单一剂量的NC中混合10mL蜂蜜，表现出更少的组织病理学变化。与对照相比，佐剂（蜂蜜）治疗组未显示肝肾中的任何组织病理学毒性。结论：虽然在2000mg / kg体重的单剂量下没有死亡率，但血清显示出毒性的显着积累。它在肝肾中产生了组织病理学毒性的迹象。观察到剂量依赖性变化。建议仅在低剂量下使用Chenduram，这是有效的。在我们以前的工作中证明，NC治疗剂量水平随着AG的NC在Wistar Albino大鼠的Rotenone诱导帕金森病中有效。

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