...
  • 主题
高级搜索  >
历史搜索 清空历史
首页> 外文期刊>MethodsX >Whole mount staining of lenses for visualization of lens epithelial cell proteins
【24h】

Whole mount staining of lenses for visualization of lens epithelial cell proteins

机译：镜片上皮细胞蛋白可视化的整体贴染色

获取原文
           
页面导航
  • 摘要
  • 著录项
  • 相似文献
  • 相关主题

摘要

Whole mount imaging of the lens allows for high spatial resolution visualization of lens epithelial structures by using small molecule fluorescent probes. However, the visualization of specific proteins in lens epithelial cells within whole lenses remains a challenge as the capsule that surrounds the lens does not allow penetration of antibodies. Here we describe a whole mount imaging method that allows us to overcome this challenge by digesting the lens capsules of paraformaldehyde fixed lenses using collagenase. This method enables the penetration of antibodies for effective visualization of proteins in the epithelium of whole lenses.?A limitation to lens whole mount imaging is the ability to visualize specific proteins as the collagen capsule surrounding the lens impedes the penetration of antibodies?This protocol helps overcome this limitation by a light collagenase digestion of the capsule of fixed lenses prior to immunostaining?This method allows for the imaging of specific proteins in the epithelium of the whole lens tissue.
机译：通过使用小分子荧光探针，透镜的整个安装成像允许高空间分辨率可视化镜头上皮结构。然而，整个镜片内的透镜上皮细胞中特定蛋白质的可视化仍然是围绕镜片的胶囊不允许抗体的胶囊的挑战。在这里，我们描述了一种全部安装成像方法，其允许我们通过使用胶原酶消化多变甲醛固定透镜的镜头胶囊来克服这一挑战。该方法能够渗透抗体在整个镜片上皮中有效可视化蛋白质。对镜头整体安装成像的限制是可视化特异性蛋白质作为镜片围绕镜片的胶囊阻碍抗体的渗透性的能力？该方案有助于通过在免疫染色之前通过固定透镜胶囊的轻质胶原酶消化克服这种限制？该方法允许在整个透镜组织的上皮中成像特异性蛋白质。

著录项

相似文献

  • 外文文献
  • 中文文献
  • 专利
获取原文

客服邮箱：kefu@zhangqiaokeyan.com

京公网安备：11010802029741号 ICP备案号：京ICP备15016152号-6 六维联合信息科技 (北京) 有限公司©版权所有

  • 客服微信

  • 服务号