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首页> 外文期刊>BMC Genomics >Comparative genome and transcriptome analyses reveal innate differences in response to host plants by two color forms of the two-spotted spider mite Tetranychus urticae
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Comparative genome and transcriptome analyses reveal innate differences in response to host plants by two color forms of the two-spotted spider mite Tetranychus urticae

机译：比较基因组和转录组分析显示，通过两种斑点蜘蛛螨Tetranychus荨麻疹的两种颜色形式对宿主植物的响应进行先天差异

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摘要

The two-spotted spider mite, Tetranychus urticae, is a major agricultural pest with a cosmopolitan distribution, and its polyphagous habits provide a model for investigating herbivore-plant interactions. There are two body color forms of T. urticae with a different host preference. Comparative genomics and transcriptomics are used here to investigate differences in responses of the forms to host plants at the molecular level. Biological responses of the two forms sourced from multiple populations are also presented. We carried out principal component analysis of transcription changes in three red and three green T. urticae populations feeding on their original host (common bean), and three hosts to which they were transferred: cotton, cucumber and eggplant. There were differences among the forms in gene expression regardless of their host plant. In addition, different changes in gene expression were evident in the two forms when responding to the same host transfer. We further compared biological performance among populations of the two forms after feeding on each of the four hosts. Fecundity of 2-day-old adult females showed a consistent difference between the forms after feeding on bean. We produced a 90.1-Mb genome of the red form of T. urticae with scaffold N50 of 12.78?Mb. Transcriptional profiles of genes associated with saliva, digestion and detoxification showed form-dependent responses to the same host and these genes also showed host-specific expression effects. Our research revealed that forms of T. urticae differ in host-determined transcription responses and that there is form-dependent plasticity in the transcriptomic responses. These differences may facilitate the extreme polyphagy shown by spider mites, although fitness differences on hosts are also influenced by population differences unrelated to color form.
机译：两斑的蜘蛛螨Tetranychus荨麻疹是一个主要的农业害虫，具有大都会分布，其复变习惯提供了一种调查食草动物植物相互作用的模型。荨麻疹有两种身体彩色形式，具有不同的主体偏好。这里使用比较基因组学和转录组科以研究在分子水平下宿主植物的形式的反应的差异。还提出了来自多种群体的两种形式的生物反应。我们对其原始宿主（共同豆类）的三个红色和三种绿色T.荨麻疹荨麻疹的转录变化进行了主成分分析，以及他们转移的三个主机：棉，黄瓜和茄子。无论其宿主植物如何，基因表达的形式存在差异。此外，当响应相同的主体转移时，在两种形式中，基因表达的不同变化是显而易见的。我们进一步比较了在喂食四个宿主中的每一个后两种形式的生物学性能。 2天老年女性的繁殖力显示在豆类喂养后的形式之间存在一致的差异。我们生产了一种90.1 MB基因组，红色形式的T.荨麻疹，带有12.78 mb的支架N50。与唾液，消化和解毒相关的基因的转录谱显示出对同一宿主的形式依赖性反应，这些基因也显示出宿主特异性的表达效应。我们的研究表明，T.荨麻疹的形式在宿主确定的转录反应中不同，并且在转录组反应中存在依赖性可塑性。这些差异可以促进蜘蛛螨显示的极端多斑，尽管宿主的适应性差异也受到与颜色形式无关的群体差异的影响。

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