• 主题
高级搜索  >
历史搜索 清空历史
首页> 外文期刊>Sensing and Bio-Sensing Research >Zepto molar miRNA-21 detection in gold Nano-islands platform toward early cancer screening
【24h】

Zepto molar miRNA-21 detection in gold Nano-islands platform toward early cancer screening

机译：Zepto Molar miRNA-21在金纳米岛平台中检测早期癌症筛查

获取原文
           
页面导航
  • 摘要
  • 著录项
  • 相似文献
  • 相关主题

摘要

MicroRNAs (miRs) as biomarkers for early cancer diagnostic, were detected through a developed ultrasensitive electrochemical biosensor containing gold Nano-islands (Au-NIs) structures. Different concentrations of miR-21 (from zepto -molar to micro range) in both standard solution and blood serum samples were measured to test the sensitivity of biosensor. A high surface-area-to-volume ratio of nanostructures embellished on the FTO electrodes increases the chance of immobilization of the thiol modified capture probe (anti-miR-21 or cap-21). The Au-nanostructures surface modification and its ability to hybridize successfully and stably anti-miR-21 and miR-21 confirmed by electrochemical impedance spectroscopy (EIS), and cyclic voltammetry (CV) electrochemical techniques. The results showed that the linear detection range of the biosensor was from 1.0 zM to 200?nM with a limit of detection (LOD) of 0.12 zM which revealed a very high sensitivity. The selectivity of the biosensor was also investigated and confirmed by detecting miR-9 and miR-486. Corresponding results revealed that the biosensor discriminated target miR-21 from non-complementary miR-21 and showed high selectivity and specificity. Our results also revealed that this platform is a promising substrate to quantitative analysis of miR-21 in physiological samples and could be used for biomedical research and point-of-care (POC) diagnosis. Straightforward and robust Au-NIs surface fabricated without surfactant and stabilizer additive, with plenty of anchors for ssDNA immobilization, as well as well-established hybridization and immobilization procedures, plus high electron transfer rate ability in comparison to bare FTO electrodes are among attractive and suitable novelty of this study.
机译：通过含有金纳米岛（AU-NIS）结构的显式的超细电化学生物传感器来检测MicroRNAS（MIRS）作为早期癌症诊断的生物标志物。测量标准溶液和血清样品中不同浓度的miR-21（来自Zepto -molar到微型）以测试生物传感器的敏感性。在FTO电极上装饰的纳米结构的高表面积到体积比增加了固定硫醇改性捕获探针的可能性（抗miR-21或CAP-21）。通过电化学阻抗谱（EIS）和循环伏安法（CV）电化学技术证实，Au-纳米结构表面改性及其杂交杂交和稳定抗miR-21和miR-21的能力。结果表明，生物传感器的线性检测范围为1.0 ZM至200≤NM，其检测极限为0.12 ZM，显示出非常高的灵敏度。还通过检测miR-9和miR-486来研究生物传感器的选择性并确认。相应的结果表明，生物传感器与非互补miR-21中的靶miR-21鉴定并显示出高的选择性和特异性。我们的研究结果还显示，该平台是对生理样品中miR-21的定量分析的有希望的基材，可用于生物医学研究和护理点（POC）诊断。没有表面活性剂和稳定剂添加剂制造的直接和稳健的Au-NIS表面，具有大量锚固SSDNA固定，以及良好的杂交和固定程序，以及与裸FTO电极相比的高电子传递速率能力是有吸引力和合适的本研究的新颖性。

著录项

相似文献

  • 外文文献
  • 中文文献
  • 专利
获取原文

客服邮箱：kefu@zhangqiaokeyan.com

京公网安备：11010802029741号 ICP备案号：京ICP备15016152号-6 六维联合信息科技 (北京) 有限公司©版权所有

  • 客服微信

  • 服务号