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Isolation of Bovine leukemia virus from cows with persistent lymphocytosis in Iraq

机译：伊拉克持续淋巴细胞症的牛牛白血病病毒的分离

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This is the first study to report on the isolation of bovine leukemia virus (BLV) from peripheral blood mononuclear cells of two cross bred cows in Iraq. The cattle were seropositive by ELISA when selected while being surveyed for the detection of BLV. Among six cows, two were cases of persistent lymphocytosis (PL). Cytopathology was characterized by the formation of multinucleated giant cells (syncytia) and cytoplasmic vacuoles. Moreover, the viruses produced clear plaques on the monolayer of the primary fetal calf kidney (FCK) cells. Inhibition of plaque formation by BLV-antisera suggested a diagnosis of BLV, which was further confirmed by PCR. Cells infected with the isolates were positive to a monoclonal antibody against the viral gp51 trans-membrane glycoprotein by immunocytochemistry. Both isolates replicated and induced cytopathic effects in bovine and human cell line cultures. Phylogenetic analysis based on partial gp51 env gene sequences revealed that Iraqi strain highly homogenous with Turkey strain (100%) and had 1% distance value with other world strains. In conclusion, this present study found that BLV-infected cattle with PL can be a source for viral isolation, and the cytopathological features of the virus infection are arranged and differ depending on the cell type. This is the first study to report on the isolation of the EBL virus in Iraq, and it provides the basis for further studies about a BLV Iraqi strain that can help control this disease.
机译：这是第一次报告从伊拉克两交叉养牛的外周血单核细胞分离牛白血病病毒（BLV）的研究。当在被调查以检测BLV时选择时，牛被ELISA血清阳性。在六头奶牛中，两种患者是持续的淋巴细胞增多率（PL）。通过形成多核巨细胞（Syncytia）和细胞质液泡，表征缩细胞病变。此外，病毒在原发性胎牛肾（FCK）细胞的单层上产生了透明的斑块。 BLV-Antisera对斑块形成的抑制表明BLV的诊断，通过PCR进一步证实。通过免疫细胞化学，用分离物感染分离物的细胞对病毒GP51跨膜糖蛋白的单克隆抗体呈阳性。两种分离株在牛和人细胞系培养物中复制和诱导细胞病变效应。基于部分GP51 ENV基因序列的系统发育分析表明，伊拉克菌株与火鸡菌株（100％）高度均匀，与其他世界菌株有1％的距离值。总之，本研究发现，具有PL的BLV感染的牛可以是病毒分离的源，并且根据细胞类型排列病毒感染的细胞病理学特征。这是第一次报告伊拉克EBL病毒的分离的研究，它为进一步研究提供了有助于控制这种疾病的BLV伊拉克菌株的基础。

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