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首页> 外文期刊>Journal of King Saud University >Responses of Enterococcus faecalis resistance and cytolysin up-regulation to nutrients in constructed mesocosms
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Responses of Enterococcus faecalis resistance and cytolysin up-regulation to nutrients in constructed mesocosms

机译：肠球菌的反应抗性和胞嘧啶上调构建中科姆营养的营养素

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Spreading of multidrug-resistant bacteria has become a growing and significant threat to environmental and public health. Our understanding of environmental variables such as nutrients which contribute to the dissemination of antibiotic resistance and pathogenesis is still limited. In this study, we operated outdoor mesocosm experiments to evaluate how nitrate and phosphate ions with different concentrations influence on an adaptation ofEnterococcus faecalisto aquatic environments.E.faecaliswere isolated from the mesocosms at 8 sampling events for 96?days to evaluate biofilm production, quorum-sensing-related genes expression, and sensitivity to oxytetracycline, erythromycin, ciprofloxacin, ampicillin, vancomycin, and chloramphenicol. Quantitative real-time PCR was used to compare mRNA levels ofE.faecalisquorum-sensing-related genes.E.faecalisisolates exhibited resistance to oxytetracycline, ampicillin, and ciprofloxacin, respectively. We observed that the biofilm production ofE.faecalisisolates on day 60 and 96 was significantly increased (p?
机译：多药抗性细菌的传播已成为对环境和公共卫生的日益增长和重大威胁。我们对环境变量的理解，例如有助于传播抗生素抗性和发病机制的营养素仍然有限。在这项研究中，我们经营了室外中科姆实验，以评估具有不同浓度的硝酸盐和磷酸盐的离子对Eterococcus Faecalisto水生环境的适应..Faecaliswere在8个采样事件中与Mesocosms分离出96次，以评估生物膜的生物膜生产，Quorum-感测相关的基因表达，对氧赤霉素，红霉素，环丙沙星，氨苄青霉素，万古霉素和氯霉素的敏感性。定量实时PCR用于比较Faecalisquorum感应相关基因的mRNA水平..Faecalisolates分别表现出对催产素，氨苄青霉素和环丙沙星的抗性。我们观察到，第60和第96天的生物膜产生的生物膜产生显着增加（P？<？0.01）。批量传感相关基因的表达明显上调（〜十倍）在富含营养的处理中的转录水平。我们的研究结果表明，硝酸盐和磷酸盐离子促进常用抗生素，增加生物膜生产和物种内部通信，这可能是水生环境的主要恢复策略。

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