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首页> 外文期刊>Journal of King Saud University >S-phase cell cycle arrest, and apoptotic potential of Echium arabicum phenolic fraction in hepatocellular carcinoma HepG2 cells
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S-phase cell cycle arrest, and apoptotic potential of Echium arabicum phenolic fraction in hepatocellular carcinoma HepG2 cells

机译：S期细胞周期停滞， echiumAlabicum 肝细胞癌Hepg2细胞酚类分数的凋亡潜力

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Plants have been used in the treatment of many diseases. The aim of the present investigation was to assess the cytotoxic potential ofEchium arabicumextracts against liver and colon cancer cells. The plant was extracted using Soxhlet apparats and ultrasound-assisted hydrolysis. The phenol and antioxidant activity was assessed using Folin–Ciocalteu and DPPH method respectively. The cytotoxicity was determined using MTT and lactate dehydrogenase release assay. The apoptosis study was conducted using DAPI, and Acridine orange/ethidium bromide and confirmed by Caspase 3/7 Fluorescence reagent, and the Cell cycle analysis was carried out using Muse Cell Analyzer.E. arabicumhad cytotoxic properties with half inhibitory concentration (IC50) values of 61.6?±?0.6, 66.4?±?0.9, 71.9?±?0.8, and 38.3?±?0.3?μg/mL against LoVo, HCT116, HuH-7 and HepG2 respectively using MTT assay. Interestingly, the phenolic fraction showed less cytotoxicity at the highest concentration tested on normal human liver (Chang) cells. Staining of HepG2 cells with DAPI and AO/EB post treatment with phenolic fraction (40 and 60?μg/mL) decreased the number of viable cells and exhibited typical features of apoptosis like blebbing of plasma membrane with an increase in the Caspase-3/7 activity. Furthermore, cell cycle study revealed thatE. arabicuminduced a S-phase arrest in HepG2 cells. The coefficient of determination (R2?=?0.997) and the Pearson’s correlation coefficient (r?=?0.998) between DPPH activity and total phenolic content was highly correlated. These results demonstrate that phenolic fraction fromE. arabicummight have a strong potential as chemopreventive agent in liver cancer.
机译：植物已被用于治疗许多疾病。本研究的目的是评估对抗肝脏和结肠癌细胞的细胞毒性潜力。使用Soxhlet Acazats和超声辅助水解提取植物。使用Folin-Ciocalteu和DPPH方法评估苯酚和抗氧化活性。使用MTT和乳酸脱氢酶释放测定测定细胞毒性。使用DAPI和吖啶/乙锭进行并通过Caspase 3/7荧光试剂证实，进行凋亡研究，使用Muse细胞分析仪进行细胞循环分析。 Arabicumhad细胞毒性特性，半抑制浓度（IC50）值为61.6Ω·α≤0.6,66.4？±0.9,71.9？±0.8和38.3？±0.3Ω·μg/ ml对LOVO，HCT116，HUH-7和HEPG2分别使用MTT测定。有趣的是，酚类馏分在正常人肝（Chang）细胞上测试的最高浓度下表现出较少的细胞毒性。用DAPI和AO / EB的HepG2细胞染色用酚类馏分（40和60Ω×mL）降低了活细胞的数量，并表现出肠膜的典型特征，如血浆膜的膨胀，随着Caspase-3的增加/ 7活动。此外，细胞周期研究揭示了瑟。 Arabicum indizE在HepG2细胞中的S相捕获。在DPPH活性和总酚醛含量之间的测定系数（R2？= 0.997）和Pearson的相关系数（R？= 0.998）高度相关。这些结果表明酚类级分frome。阿拉伯式ummight在肝癌中具有强烈的化学预防剂。

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