• 主题
高级搜索  >
历史搜索 清空历史
首页> 外文期刊>Journal of King Saud University >Escin induces apoptosis in ovarian cancer cell line by triggering S-phase cell cycle arrest and p38 MAPK/ERK pathway inhibition
【24h】

Escin induces apoptosis in ovarian cancer cell line by triggering S-phase cell cycle arrest and p38 MAPK/ERK pathway inhibition

机译：Escin通过触发S相细胞周期停滞和P38 MAPK / ERK途径抑制诱导卵巢癌细胞系中凋亡

获取原文
           
页面导航
  • 摘要
  • 著录项
  • 相似文献
  • 相关主题

摘要

BackgroundOvarian cancer (OC), is a common malignant tumors in the female reproductive system with the increased mortality rate. The occurrence of OC is elevating rapidly in recent decades. Escin is a triterpene saponin reported to possess the significant biological activities.ObjectiveThe current investigation focused to address thein vitroanticancer actions of escin against the OC A2780 cells through the inhibition of p38 MAPK/ERK pathway.MethodologyThein vitroantioxidant potential of escin was examined using different free radicals scavenging assays like reducing power, DPPH, and superoxide radicals. Escin treated Vero and A2780 cell’s viability was investigated by MTT assay. The lipid peroxidation, antioxidants SOD and GSH was quantified in the escin treated A2780 cells were by standard methods. The effect of escin on the ROS accumulation, MMP, and apoptotic cell death in A2780 cells was scrutinized by respective fluorescence staining assays. The cell cycle transition was studied by flow cytometry and the expressions of p38 MAPK/ERK molecules were investigated using RT-PCR analysis.ResultsEscin possessed the appreciable reducing power and scavenged the DPPH and superoxide radicals, which proves the antioxidant capacity of escin. The viability of A2780 cells was remarkably suppressed by the escin and it did not possessed toxicity to the normal Vero cells. Escin improved the lipid peroxidation and suppressed the SOD and GSH levels in the A2780 cells. The status of MMP was substantially decreased and the ROS and apoptotic cells were drastically elevated in the escin administered A2780 cells. Escin treatment notably suppressed the p38 MAPK/ERK signaling axis in the A2780 cells.ConclusionThe findings of this investigation have revealed that the escin has demonstrated the potentin vitroanticancer actions against the OC A2780 cells.
机译：背景技术癌症（OC）是女性生殖系统中的常见恶性肿瘤，死亡率增加。 OC的发生近几十年来迅速提升。谢内是一个据报道，普罗顿蛋白是一个具有重要的生物学活动。目前的调查集中于通过抑制P38 Mapk / Erk途径来解决Escin对OC A2780细胞的族族族族族对抗族A2780细胞的影响。使用不同的自由基检查eSCIN的亚申德内乙酸亚毒性毒性潜力清除测定等降低功率，DPPH和超氧化物自由基。通过MTT测定研究了Escin治疗的Vero和A2780细胞的活力。通过标准方法，在ESCIN处理A2780细胞中定量脂质过氧化，抗氧化剂SOD和GSH是通过标准方法的。 Escin对A2780细胞中ROS累积，MMP和凋亡细胞死亡的影响是通过各自的荧光染色测定仔细仔细仔细筛选。通过流式细胞术研究细胞周期转变，使用RT-PCR分析研究了P38 MAPK / ERK分子的表达。培养型素具有可观的降低功率并清除DPPH和超氧化物自由基，证明escin的抗氧化能力。 ESCIN显着抑制A2780细胞的可行性，并且对正常的VERO细胞没有具有毒性。 Escin改善了脂质过氧化并抑制了A2780细胞中的SOD和GSH水平。 MMP的状态显着降低，并且ROS和凋亡细胞在ESCIN施用的A2780细胞中急性升高。 Escin治疗显着抑制了A2780细胞中的P38 MAPK / ERK信号轴。结论该调查的结果表明，Escin已经证明了对OC A2780细胞的吐痰族族族常规作用。

著录项

相似文献

  • 外文文献
  • 中文文献
  • 专利
获取原文

客服邮箱：kefu@zhangqiaokeyan.com

京公网安备：11010802029741号 ICP备案号：京ICP备15016152号-6 六维联合信息科技 (北京) 有限公司©版权所有

  • 客服微信

  • 服务号