CuO/NiO bimetallic nanocomposite: A facile DNA assisted synthetic approach and evaluation of bio efficacy

Sona G. Krishnan; Deepak Nand; Seena Sivadas; Ahmed Alfarhan; Karnan Muthusamy

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CuO/NiO bimetallic nanocomposite: A facile DNA assisted synthetic approach and evaluation of bio efficacy

机译：CuO / NiO双金属纳米复合材料：容易DNA辅助合成方法和生物效能评估

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In this present work, a simple chemical co-precipitation method was used to synthesize deoxyribonucleic acid (DNA) capped CuO-NiO bimetallic nanocomposite. The morphology and structure related properties of the samples were analyzed using Energy Dispersive X-Ray Analysis, X-Ray Diffraction, Transmission Electron Microscopy and Scanning Electron Microscopy. Widely used Agar well diffusion method evaluated the antimicrobial activity of the sample. DPPH and FRAP assays were applied to test the antioxidant activity of the sample. Average crystallite size of 16.218?nm NiO and 15.871?nm CuO were confirmed within CuO- NiO bimetallic oxide nanoparticles by means of XRD technique. EDAX studies confirmed the purity of the sample. The grain size obtained from TEM studies matches with the XRD results. The free radical scavenging activity of sample was higher in lower concentrations, viz. 0.1, 1, 5 and 10?μg/ml of the sample and activity decreased after that. The IC50 value in FRAP assay shows the potential of this mixed metal oxide nanoparticle in radical scavenging. In agar well cut method, the sample showed moderate antibacterial activity againstMycobacterium smegmatisandSalmonella typhimurium. The results also showed that the CuO-NiO bimetallic nanoparticles are biologically compatible, environmentally benign and economical material having potential applications in biomedical industry.

机译：在本工作中，使用简单的化学共沉淀法合成脱氧核糖核酸（DNA）Cuo-NiO双金属纳米复合材料。使用能量分散X射线分析，X射线衍射，透射电子显微镜和扫描电子显微镜分析样品的形态和结构相关性能。广泛使用的琼脂扩散方法评估样品的抗微生物活性。施用DPPH和FRAP测定以测试样品的抗氧化活性。通过XRD技术在Cuo-NiO二金属氧化物纳米颗粒中确认了平均微晶尺寸为16.218≤NIO和15.871·NM CUO。 edax研究证实了样品的纯度。从TEM研究获得的晶粒尺寸与XRD结果相匹配。样品的自由基清除活性较低浓度，浓度高。在此之后，0.1,1,5和10？μg/ ml样品和活性降低。 Frap测定中的IC 50值显示出这种混合金属氧化物纳米颗粒在激进清除的潜力。在琼脂良好切割方法中，样品显示患有中度抗菌活性，对施霉杆菌菌氏菌菌杆菌氏菌。结果还表明，CuO-NiO双金属纳米粒子是生物医学行业具有潜在应用的生物兼容性，环境良性和经济材料。

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《Journal of King Saud University》 |2022年第1期|共7页
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Sona G. Krishnan; Deepak Nand; Seena Sivadas; Ahmed Alfarhan; Karnan Muthusamy;
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NanocompositesCapping agentsDNAAntimicrobialAntioxidant;

机译：纳米复合施用剂addnaInicrobialantioxidant.;

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CuO/NiO bimetallic nanocomposite: A facile DNA assisted synthetic approach and evaluation of bio efficacy

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