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外文期刊>Journal of King Saud University
>Isolation and characterisation of Streptomyces sp. Strain GLD25 with antimicrobial and antioxidant effects from Gueldaman cave (GLD1), Akbou-Algeria
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Isolation and characterisation of Streptomyces sp. Strain GLD25 with antimicrobial and antioxidant effects from Gueldaman cave (GLD1), Akbou-Algeria
ObjectivesThe present study aimed to isolate potential antimicrobial and antioxidantStreptomyces spfrom cave areas ofAlgeria.MethodsStreptomycessp.GLD25 was identified by biochemical, physiological and molecular analysis. The antimicrobial activity has been carried out by well diffusion method, microplates method has been used to perform the MIC values of the organic extracts. The antioxidant capacity of the extract was tested by DPPH and FRAP methods. GC–MS analysis was used to study the chemical profile of the crude active extract.ResultsThe novel strainStreptomycessp. GLD25 was isolated from Gueldaman cave in Algeria showed potential antioxidant and antimicrobial properties. According to the results of morphological, biochemical characteristics and complete 16S rRNA sequence, the strain revealed 99.71% of similarity withStreptomyces cyaneofuscatusstrain WKFF71.The significant inhibition zone of the crude extract and MIC value were 30?mm and 0,015?mg/mL respectively againstStaphylococcus aureusandBacillus subtilis. In addition, the extract showed good antioxidant activity with IC50?=?20,12?mg/mL?±?2,05 for DPPH and IC50?=?8,80?mg/mL?±?0,2 for ferric reducing activity. The fundamental metabolites of the active extract were identified using GC–MS were Diisooctyl-phthalate, 6-hydroxy-heptanoic acid, Hexadecanoic acid, Benzeneacetic acid and 3-(3,5-di-tert-butyl-4-hydroxyphenyl)propionic acid.ConclusionOverall, theStreptomycessp.GLD25 isolated from Gueldamen cave have the potential to produce antimicrobials and antioxidants compounds with important biotechnological and medical applications.
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