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首页> 外文期刊>Journal of King Saud University >Biogenic synthesis of ZnO and NiO nanoparticles mediated by fermented Cocos nucifera. (L) deoiled cake extract for antimicrobial applications towards gram positive and gram negative pathogens
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Biogenic synthesis of ZnO and NiO nanoparticles mediated by fermented Cocos nucifera. (L) deoiled cake extract for antimicrobial applications towards gram positive and gram negative pathogens

机译：ZnO和NiO纳米粒子的生物合成由发酵的Cocos Nucifera介导的。 （l）糊化的蛋糕提取物用于抗微生物应用朝向克阳性和革兰氏阴性病原体

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BackgroundCocos nucifera(L) is a widespread tall plant and found to havelot of important pharmacological applications and it is very less toxic in nature. The water of the endocarp has high antioxidant activity, and its fiber was reported to have antibacterial, ant parasitic and anti-inflammatory properties. The oil derived from its edible parts have been used in cooking, in the preparation of the soaps and cosmetics etc. The water within can be processed to produce alcohol. Once the endocarp was crushed and the extract were removed it can used as a feed material and as a natural organic fertilizer. Herein, we report the novel method of utilizing the residue of the deoiled seed cake ofCocos nucifera(L) for preparing the metal oxides as a reducing agent and to test its antimicrobial level against human pathogens.MethodsThe method adopted in this investigation is the fermentation process of preparing theCocos nucifera(L) extract by utilizing the water as the medium for the extraction process under room temperature.ResultsStructural nature and morphology of the synthesized samples were carried out using XRD and SEM analysis and the results revealed that ZnO belongs to hexagonal shape with primitive lattice cell arrangement and NiO had cubic structure with FCC lattice arrangements. The results of the average crystalline size of prepared nanoparticles using XRD and SEM were alike. Molecular bonding and stability were analyzed using FTIR instrumentation based on UV absorption andTauc plotthe bandgap of ZnO and NiO was calculated as 3.15?eV and 3.64?eV. Elemental composition was identified using EDAX analysis. Antibacterial assay was carried out against human pathogens includingS.aureus, B.subtilis, E.coliandK.pneumoniaein comparison to the standard drug Gentamycin and the synthesized specimens ZnO and NiO showed the moderate activity with the zone of inhibition of about of 8?mm, 10?mm, 12?mm and 8?mm against the human pathogens.ConclusionOur results showed that the antimicrobial properties of theCocus nucifera(L) mediated ZnO and NiO have moderate activity against the standard drug Gentamycin, three different doses were tested against the disease causing pathogens includingS.aureus, B.subtilis, E.coliandK.pneumoniae.The zone of inhibition was found to be minimum. This may be attributed to the reason that even after the removal of the edible compounds from the seed ofCocos nucifera(L), the residue or the waste product found to have a moderate antimicrobial function against pathogens.
机译：Backgroundcocos Nucifera（L）是一种广泛的高大植物，发现重要的药理学应用，并且本质上的毒性极低。 endocarp的水具有高抗氧化活性，并且据报道其纤维具有抗菌，蚂蚁寄生和抗炎特性。源自可食用部件的油已用于烹饪，在制备肥皂和化妆品等中。可以加工水中的水以产生醇。一旦粉碎内圆锥，就拔掉了提取物，它可以用作进料材料和天然有机肥。在此，我们报告了利用CocosNucifera（L）的废籽饼残余物的新方法，用于制备金属氧化物作为还原剂，并测试其对人类病原体的抗微生物水平。本研究中采用的方法是发酵过程通过利用水作为室温下的提取过程的培养基制备Thecocos Nucifera（L）提取物。使用XRD和SEM分析进行合成样品的细胞结构性质和形态，结果表明，ZnO属于六边形形状原始格子单元布置和NIO具有与FCC晶格布置的立方结构。使用XRD和SEM的制备纳米颗粒的平均结晶尺寸的结果是相似的。使用基于UV吸收的FTIR仪器分析分子键合和稳定性，并且ZnO和NiO的带隙计算为3.15？EV和3.64 eV。使用EDAX分析鉴定元素组合物。对人病原体进行抗菌测定。用标准药物庆大霉素和合成标本ZnO和NiO与标准药物蛋白蛋白和合成标本ZnO和NiO的抗菌测定进行抗菌测定，表现出与抑制区域约为8ΩΩmm的中等活动， 10？mm，12？mm和8？mm对人病原体。结果表明，Nucifera（L）介导的ZnO和NiO的抗菌性质对标准药物庆大霉素具有中等的活性，对该疾病进行了三种不同的剂量测试了三种不同的剂量导致病原体包括.Aureus，B.subtilis，E.coliandk.pneumoniae。发现抑制区是最小的。这可能归因于甚至在从Nucifera（L）种子中除去可食用化合物之后，发现残留物或发现对病原体具有中等抗微生物功能的废物。

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